Aghajanian, "Melatonin: deacetylation to 5-methoxytryptamine by liver but not brain aryl acylamidase
," Journal of Neurochemistry, vol.
The two types of cholinesterases (ChE) are present in vertebrates; AChE and BChE exhibit an aryl acylamidase
activity (AAA), which is effectively inhibited by cholinergic and serotonergic agents (ACh, specific anticholinesterase drugs and serotonin) [50, 51].
Plasma paracetamol concentrations were determined with an enzymatic, colorimetric assay that uses the enzyme acylamidase
to hydrolyse paracetamol to p-aminophenol (Cambridge Life Sciences Enzyme Assay Kit, in an Architect C8000 Analyser, Abbott Diagnostics, Illinois, USA).
Its particular effectiveness is due to the high level of acylamidase
expression in a rice plant that allows it to detoxify PRN, whereas common grass-type weeds lack this enzyme and are killed by this herbicide (Matsunaka 1968).
activity in human erythrocyte, plasma and blood in pesticide (organophosphates and carbamates) poisoning.
In our automated enzymatic assay for acetaminophen, samples are diluted with water and then mixed with reconstituted enzyme reagent (aryl acylamidase
in Tris-HCl buffer, pH 8.6).
We routinely assay ACET by an enzymatic assay (GDS enzymatic acetaminophen, GDS Diagnostics) based on the hydrolysis of ACET by acylamidase
to yield p-aminophenol and acetate.