The nine target genes were acyl-CoA synthetase
long-chain family member 6 (ACSL6), apolipoprotein A-I (APOA1), PLTP, GK, CPT2, aquaporin 7 (AQP7), acyl-Coenzyme A oxidase 1 palmitoyl (ACOX1), ACOX2, and FABP4.
Primer sequences for acetyl-CoA acyltransferase (ACAA2), long-chain acyl-CoA dehydrogenase (ACADL), acyl-CoA synthetase (ACSL1), very long chain acyl-CoA dehydrogenase (ACADVL), carnitine palmitoyltransferase 1B (CPT1B), enoyl-CoA hydratase (ECH1), hydroxyacyl-CoA dehydrogenase (HADHA), pyruvate dehydrogenase kinase (PDK4), PGC-1[alpha], PGC-1[beta], PPAR[alpha], glutamate dehydrogenase, and 18S ribosomal RNA are available in Supplemental Material, Table S1 (http://dx.
Conversely, ACSL3, which encodes an acyl-CoA synthetase enzyme involved in FA biosynthesis, was down-regulated.
Distinct transcriptional regulation of long-chain acyl-CoA synthetase isoforms and cytosolic thioesterase 1 in the rodent heart by fatty acids and insulin.
This requires their activation by long chain fatty acyl-CoA synthetase
, on the outer mitochondrial membrane, to generate long chain fatty acyl-CoA.
In this study, DR did not affect the expression of several genes in the LM for lipid metabolism (adipose triglyceride lipase, acyl-CoA synthetase long-chain family member, glycerol-3-phosphate acyltransferase, and hydroxyacyl-Coenzyme A dehydrogenase) and fatty acid uptake (fatty acid translocase and lipoprotein lipase) at both P1 and P2.
Primers for real time-PCR analysis Gene category Gene name GenBank ID Primer Growth hormone Growth hormone receptor NM_176608 Forward signaling (GHR) Reverse Signal transducer and NM_174617 Forward activator of Reverse transcription 5B (STAT5B) Insulin-like growth NM_001077828 Forward factor (IGF-1) Reverse Androgen receptor NM_001244127 Forward Reverse Myostatin NM_001001525 Forward Reverse Lipogenesis Fatty acid synthase NM_001012669 Forward (FASN) Reverse Fatty acid binding BT10868 Forward protein 4 (FABP4) Reverse Lipid Adipose triglyceride NM_001046005 Forward metabolism lipase (ATGL) Reverse Acyl-CoA synthetase NM_001076085.
1711 gonadotropin-regulated long chain acyl-CoA synthetase
When SARA was induced, acyl-CoA synthetase (ACS) and cytochrome b5 involved in lipid metabolism were up-regulated, phosphoenolpyruvate carboxykinase (PEPCK) involved in glucose metabolism was downregulated, while enolase was up-regulated.
Acyl-CoA synthetase is a crucial enzyme that facilitates the uptake and permits the metabolism of intracellular lipids.
Recent studies report that ACSL1 is the predominant acyl-CoA synthetase of lactating bovine mammary tissues (Rudolph et al.
023 (NADP+), soluble Triacylglyceride synthesis acyl-CoA synthetase long-chain ACSL1 0.