Generally speaking, a 10% active sulfate
system will require about 1-2% of alkanolamide and 1-3% sodium chloride to reach or exceed a viscosity of 10,000 cps.
reduction in Postgate's Medium B is evidenced by the production of Fe[S.sub.2] that appears as a black precipitate in the growth medium.
Toad skin is capable of active sulfate
influx from a Ringer's solution containing 1 mmol [1.sup.-1] [SO.sub.4], using an anion exchange mechanism (Larsen and Simonsen, 1988).
Bidigare and Biggs (1980) determined that active sulfate
loss from the ctenophore Beroe cucumis could be compensated by isosmotic chloride exchange.
The co-substrate and active sulfate
donor was radiolabeled 3'-phosphoadenosine 5'-phosphosulfate (([sup.35]S)PAPS) 7[Mu]M (2145 dpm/pmol) in the presence of pyrophosphatase and sulfatase inhibitors and 10 mM [Mg.sup.2+] buffered at pH 6.3.