Zhang's team unexpectedly discovered an active mutant form of human Chk1, which is also a non-natural form of this gene.
Remarkably, the research team discovered that when expressed in cancer cells, this active mutant form of Chk1 permanently stopped cancer cell proliferation and caused cell death in petri dishes even without the addition of any chemotherapeutic drugs.
Additionally, we observed that mice possessing constitutively active mutant
Tarceva (erlotinib) Is Effective In Blocking Downstream Activation of AKT and ERK In Primary Human Tumor Explant Xenografts Containing Constitutively Active Mutant
1a]AR with enhanced green fluorescent protein (EGFP) or a constitutively active mutant
To address this, transgenic mice were generated which over-express the [alpha]lA-AR with enhanced green fluorescent protein (EGFP) or constitutively active mutant
By transfection of cells with dominant negative or constitutively active mutants
of cells we will study the effect of the kinases on the activity or phosphorylation of substrates, and on cell growth etc.
Knowles says he expects that running the most active mutants
through more mutation-and-selection cycles should lead to enzymes that show even more activity.