The selected hyper active mutant
strains were subjected to further screening on the basis of partially purified SK enzyme activity (Table II).
A potential weakness of this study is the use of a constitutive active mutant
of ER[alpha] to facilitate crystallography, which Arao suggests might alter the chemical-receptor interactions.
In this study, we used a constitutively active mutant of Ras to induce oncogene-induced senescence and hydrogen peroxide ([H.sub.2][O.sub.2]) to induce oxidative stress-induced senescence in human umbilical vein endothelial cells (HUVECs).
Infection with a replication-defective adenovirus that expresses a constitutively active mutant of mouse Ras (AdRas12V) was reported previously .
Zhang's team unexpectedly discovered an active mutant
form of human Chk1, which is also a non-natural form of this gene.
Additionally, we observed that mice possessing constitutively active mutant
(CAM) [[alpha].sub.1A]ARs produced a significantly greater latency to myclonic jerk and tonic clonic state compared to wild-type in flurothyl-induced seizures.
(2000) also adapted the enzymatic diffusion zone method to select the active mutant
for glucose oxidase activity.
To address this, transgenic mice were generated which over-express either the [[alpha].sub.1a]AR with enhanced green fluorescent protein (EGFP) or a constitutively active mutant
To address this, transgenic mice were generated which over-express the [alpha]lA-AR with enhanced green fluorescent protein (EGFP) or constitutively active mutant
To further optimize TNXI's potential industrial utility random mutagenesis using the TNXI V185T-encoding gene as the template was performed and two highly active mutants
3A2 (V185T/L282P) and 1F1 (V185T/L282P/F186S) were obtained.
Determination of cellular roles of Mnk1/2.By transfection of cells with dominant negative or constitutively active mutants
of cells we will study the effect of the kinases on the activity or phosphorylation of substrates, and on cell growth etc..
Knowles says he expects that running the most active mutants
through more mutation-and-selection cycles should lead to enzymes that show even more activity.