The selected hyper active mutant
strains were subjected to further screening on the basis of partially purified SK enzyme activity (Table II).
A potential weakness of this study is the use of a constitutive active mutant
of ER[alpha] to facilitate crystallography, which Arao suggests might alter the chemical-receptor interactions.
In this study, we used a constitutively active mutant of Ras to induce oncogene-induced senescence and hydrogen peroxide ([H.sub.2][O.sub.2]) to induce oxidative stress-induced senescence in human umbilical vein endothelial cells (HUVECs).
Infection with a replication-defective adenovirus that expresses a constitutively active mutant of mouse Ras (AdRas12V) was reported previously .
Zhang's team unexpectedly discovered an active mutant
form of human Chk1, which is also a non-natural form of this gene.
Additionally, we observed that mice possessing constitutively active mutant
(CAM) [[alpha].sub.1A]ARs produced a significantly greater latency to myclonic jerk and tonic clonic state compared to wild-type in flurothyl-induced seizures.
(2000) also adapted the enzymatic diffusion zone method to select the active mutant
for glucose oxidase activity.
To address this, transgenic mice were generated which over-express either the [[alpha].sub.1a]AR with enhanced green fluorescent protein (EGFP) or a constitutively active mutant
To address this, transgenic mice were generated which over-express the [alpha]lA-AR with enhanced green fluorescent protein (EGFP) or constitutively active mutant
To further optimize TNXI's potential industrial utility random mutagenesis using the TNXI V185T-encoding gene as the template was performed and two highly active mutants
3A2 (V185T/L282P) and 1F1 (V185T/L282P/F186S) were obtained.
Knowles says he expects that running the most active mutants
through more mutation-and-selection cycles should lead to enzymes that show even more activity.