A potential weakness of this study is the use of a constitutive active mutant
of ER[alpha] to facilitate crystallography, which Arao suggests might alter the chemical-receptor interactions.
Zhang's team unexpectedly discovered an active mutant form of human Chk1, which is also a non-natural form of this gene.
Remarkably, the research team discovered that when expressed in cancer cells, this active mutant form of Chk1 permanently stopped cancer cell proliferation and caused cell death in petri dishes even without the addition of any chemotherapeutic drugs.
Additionally, we observed that mice possessing constitutively active mutant
2000) also adapted the enzymatic diffusion zone method to select the active mutant
for glucose oxidase activity.
1a]AR with enhanced green fluorescent protein (EGFP) or a constitutively active mutant
To address this, transgenic mice were generated which over-express the [alpha]lA-AR with enhanced green fluorescent protein (EGFP) or constitutively active mutant
To further optimize TNXI's potential industrial utility random mutagenesis using the TNXI V185T-encoding gene as the template was performed and two highly active mutants
3A2 (V185T/L282P) and 1F1 (V185T/L282P/F186S) were obtained.
By transfection of cells with dominant negative or constitutively active mutants
of cells we will study the effect of the kinases on the activity or phosphorylation of substrates, and on cell growth etc.
Knowles says he expects that running the most active mutants
through more mutation-and-selection cycles should lead to enzymes that show even more activity.