Combination of Trolox and EDTA partly improved frozen-thawed sperm motility and reduced the spontaneous acrosomal reaction
This might reflect an increase of acrosomal volume, which could be related to the acrosomal reaction
Cholesterol and zinc usually stabilize sperm cell membrane and must be removed during capacitation to enable acrosomal reaction
and penetration of the ova.
Manipulation of cord structures is not associated with the production of ASA (20) and even the effect of ASA on acrosomal reaction
has been challenged in some studies (21).
At this point you might be wondering how binding of the sperm to the zona pellucida induces the morphologic changes associated with the acrosomal reaction
Induction of the acrosomal reaction
in mouse spermatozoa by the solution of pellucid zone from alpaca (Lama pacos L.
The second, third and fourth steps of this process, respectively, are the acrosomal reaction
, the sperm cell and egg cell adhesion through the vitelline envelope, and the actual membrane contact between the two cells.
0 mM)] were thawed and evaluated under phase-contrast microscope (40 A-) for sperm motility whereas sperm viability plasma membrane integrity and acrosomal integrity were assessed by the supravital staining hypo-osmotic swelling test (HOST) and normal acrosomal reaction
7) These glycoconjugates are involved in spermatogenesis, (2) capacitation, and acrosomal reaction
After incubation, sperm swim through the egg jelly coat and become bound to the vitelline envelope within 30 sec, followed by an acrosomal reaction
at 1 min.
1991) have reported that the maturation condition of the nucleus and cytoplasm of follicular oocytes plays a decisive role in the growth of in vitro fertilized oocytes after fertilization, and cumulus cells mediate an important effect on acrosomal reaction
of sperms, in vitro fertilization and the growth of fertilized oocytes in pigs.
F-actin pre acrosomal reaction
denotes the amount of filamentous actin present in unreacted sperm visualized by fluorescent staining and electron microscopy.