In general, when spermatozoa reach the oocyte, they initiate the acrosome reaction and release an enzyme called acrosin
, which degrades the oocyte ZP to allow spermatozoa to penetrate into the oocyte cytosol, where the fertilization is finally completed.
Acrosin, the most widely used acrosomal enzyme associated with the acrosome of all mammalian spermatozoa was assessed by the method of Bhattacharya and Zeneveld (1978), Bhattacharya et al.
The mechanism of action seems to be by disrupting the plasma membrane integrity as they caused drastic inhibition on sperm membrane specific enzymes like acrosin and 5' nuleotidase.
Release of acrosin and acrosin inhibitor from human spermatozoa.
Two satisfactory methods for purification of human acrosin.
A basic 18-amino acid peptide contain the polysulfate-binding domail responsable for activation of the boar proacrosin/ acrosin system.
Spartiumjunceum Reduced rate of fertility and Broom acrosin enzyme activity.
Kennedy WP, Vander Ven HH, Straus JW, Bhattacharya AK, Waller DP, Zaneveld LJD, Polakoski KL (1983) Gossypol inhibition of acrosin and proacrosin and oocyte penetration by human spermatozoa.
antibodies present in the sera of infertile women inhibit spermzona pellucida binding through protease activity (8).
Our observation of a positive association with sperm DNA damage, as measured by Tail%, may also be consistent with previous reports that BP may be a suitable vaginal contraceptive because of its ability to inhibit acrosin
(an enzyme that aids sperm penetration into the oocyte during the fertilization process), most likely by damaging the sperm membrane (Song et al.
Demonstration of proacrosin and quantitation of acrosin
in ejaculated human spermatazoa.