acridine orange stain

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acridine orange stain,

a fluorochrome stain used to detect bacteria and fungi in blood cultures, cerebrospinal fluid, buffy coats, and corneal scrapings. The fluorochrome inserts into nucleic acid and fluoresces orange when attached to bacteria or fungi.


a dibenzopyridine compound used in the synthesis of dyes and drugs. Derivatives of acridine are successful as antibacterial agents, finding their principal use as local antiseptics. They were popular at one time as antibabesial and trypanocidal agents. See also acriflavine, proflavine.

acridine orange stain
binds nonspecifically to nucleic acids, proteins, polysaccharides and glycosaminoglycans. Together with fluorescent microscopy, it is reportedly more sensitive than conventional staining methods for demonstrating Mycoplasma haemofelis in blood smears.