acridine orange


Also found in: Acronyms, Encyclopedia, Wikipedia.

ac·ri·dine or·ange

(ak'ri-dēn ōr'enj), [C.I. 46005]
3,6-bis(dimethylamino)acridine hydrochloride; a basic fluorescent dye useful as a metachromatic stain for nucleic acids; also used in screening cervical smears for abnormal and malignant cells, where unusual amounts of DNA and RNA occur during proliferation and in tumors (DNA fluoresces yellow to green; RNA fluoresces orange to red).
Farlex Partner Medical Dictionary © Farlex 2012
An immunologic fluorochrome—fluorescent dye—that nonspecifically binds to RNA (red fluorescence), DNA (green fluorescence), proteins, polysaccharides, glycosaminoglycans
Toxicology AO is carcinogenic, causing mutations by intercalating itself within replicating DNA causing insertional or deletional (‘frame shift’) mutations; AO is not routinely used in histologic laboratories
Segen's Medical Dictionary. © 2012 Farlex, Inc. All rights reserved.

ac·ri·dine or·ange

(ak'ri-dēn awr'ănj)
[CI 46005] A basic fluorescent dye useful as a metachromatic stain for nucleic acids; also used in screening cervical smears for abnormal and malignant cells.
Medical Dictionary for the Health Professions and Nursing © Farlex 2012
References in periodicals archive ?
Sofuni, "The micronucleus assay with rodent peripheral blood and acridine orange supravital staining," in Chromosomal Alterations, G.
A double-blinded comparison of in situ TUNEL and aniline blue versus flow cytometry acridine orange for the determination of sperm DNA fragmentation and nucleus decondensation state index.
Another problem we observed was the presence of necrosis or hemorrhage when the acridine orange did not stain the tissue, resulting in an inability to visualize the tissue architecture.
Blood smear examination was carried out both by conventional Giemsa staining technique and Acridine orange staining technique.
Acridine orange staining indicated that the ER stress inhibitor suppressed autophagic vacuolation induced by P.
(c) Cell death visualized by acridine orange and ethidium bromide staining.
Additionally, we selected acridine orange (AO) and 4S green plus nucleic acid stain (4S Green) as the probe, which can intercalate into the base pairs of double-stranded DNA, and the fluorescence intensity is enhanced, and they offer lower toxicity, higher stability, and convenience of using [27].
"Differential Staining of DNA and RNA in intact cells and isolated cell nuclei with acridine orange" Methods in Cell Biology 33: 285 - 98 (1990)
Mast Ceiis in Modified Thionin/ Acridine Orange Stain (100X)