acridine orange


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ac·ri·dine or·ange

(ak'ri-dēn ōr'enj), [C.I. 46005]
3,6-bis(dimethylamino)acridine hydrochloride; a basic fluorescent dye useful as a metachromatic stain for nucleic acids; also used in screening cervical smears for abnormal and malignant cells, where unusual amounts of DNA and RNA occur during proliferation and in tumors (DNA fluoresces yellow to green; RNA fluoresces orange to red).
An immunologic fluorochrome—fluorescent dye—that nonspecifically binds to RNA (red fluorescence), DNA (green fluorescence), proteins, polysaccharides, glycosaminoglycans
Toxicology AO is carcinogenic, causing mutations by intercalating itself within replicating DNA causing insertional or deletional (‘frame shift’) mutations; AO is not routinely used in histologic laboratories

ac·ri·dine or·ange

(ak'ri-dēn awr'ănj)
[CI 46005] A basic fluorescent dye useful as a metachromatic stain for nucleic acids; also used in screening cervical smears for abnormal and malignant cells.
References in periodicals archive ?
Sofuni, "The micronucleus assay with rodent peripheral blood and acridine orange supravital staining," in Chromosomal Alterations, G.
A double-blinded comparison of in situ TUNEL and aniline blue versus flow cytometry acridine orange for the determination of sperm DNA fragmentation and nucleus decondensation state index.
Another problem we observed was the presence of necrosis or hemorrhage when the acridine orange did not stain the tissue, resulting in an inability to visualize the tissue architecture.
Giemsa versus acridine orange staining in the fish micronucleus assay and validation for use in water quality monitoring.
Blood smear examination was carried out both by conventional Giemsa staining technique and Acridine orange staining technique.
Acridine orange staining indicated that the ER stress inhibitor suppressed autophagic vacuolation induced by P.
(c) Cell death visualized by acridine orange and ethidium bromide staining.
Additionally, we selected acridine orange (AO) and 4S green plus nucleic acid stain (4S Green) as the probe, which can intercalate into the base pairs of double-stranded DNA, and the fluorescence intensity is enhanced, and they offer lower toxicity, higher stability, and convenience of using [27].
"Differential Staining of DNA and RNA in intact cells and isolated cell nuclei with acridine orange" Methods in Cell Biology 33: 285 - 98 (1990)
Mast Ceiis in Modified Thionin/ Acridine Orange Stain (100X)