acridine


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Related to acridine: acridine dyes

anthracene

 [an´thrah-sēn]
a crystalline hydrocarbon, C14h10, from coal tar, used in making dyes.

ac·ri·dine

(ak'ri-dēn),
A dye, dye intermediate, and antiseptic precursor (for example, 9-aminoacridine, acriflavine, proflavine hemisulfate) derived from coal tar and irritating to skin and mucous membranes; a potent mutagen.
Synonym(s): dibenzopyridine

acridine

A dye molecule capable of causing a FRAME-SHIFT MUTATION in DNA by interposing between adjacent base pairs. Acridine orange stains nucleic acid so that under ultraviolet light DNA appears green and RNA appears orange.
References in periodicals archive ?
All fluorescence observations were made with a Zeiss Standard 14 photomicroscope with epifluorescence system equipped with an automatic C 35 camera loaded either with Kodak Gold 100 ASA film, for slides stained with acridine orange (AO) and ethidium bromide (EB), or with Kodak Ultra 400 ASA, for slides strained with JC-1 and Annexin V-FITC.
The test of fluorescence with acridine orange, based on the determination of the relation between green orthocromatic fluorescence (normal) and red metachromatic (altered) chromatin, is an adequate test to measure effects of chemical agents on male gametes (Andreetta et al., 1995; Evenson et al., 2002).
The effect of washing, frequency of washing, heating of tampon solution and concentration of acridine orange was compared using non parametric Mann-Whitney test.
Moreover, fluorescence microscopy employing double staining with Acridine Orange/Bromide Ethidium (OA/BE) (Figure 3) revealed that no significant cell death was observed in the untreated control group (medium), whereas all types of propolis extract (50 [micro]g/mL), as well as the positive control (Amph.
Mozhenok, "Acridine orange accumulation in acid organelles of normal and vacuolated frog skeletal muscle fibres," Cell Biology International, vol.
Thus, the sensitivity and specificity of these antibodies are clearly maintained after the use of BABB as a clearing agent and SYTOX Green or acridine orange as a fluorescent nuclear stain.
Dimethyl sulfoxide (DMSO), fetal bovine serum (FBS), penicillin-streptomycin, propidium iodide, acridine orange, trypsin-EDTA, and MTT powder were purchased from Sigma Chemical Co.
Currently, different types of cyotchemical tests such as aniline blue, toluiden blue, chromomycine A3 and acridine orang stainings, sperm chromatin dispersion (SCD), sperm chromatin structure assay (SCSA), comet assay and TUNEL assay are available for sperm DNA and chromatin evaluation and each of them has its own advantages and disadvantages (2).
In previous reports, different dyes such as Hoechst 33258 [11], acridine orange [12], thiazole orange [13], or hydroethidine [14] were used to determine parasitemia in cultures of Plasmodium falciparum by FACS.
Cells were stained with 10-n-nonyl acridine orange (NAO) for 30 min, washed with PBS three times and fixed with 1 per cent paraformaldehyde at 48 or 72 h post-irradiation and were analyzed by flow cytometry (23).