Slit-skin smear from the earlobe did not reveal
acid-fast bacilli.
The chances of
acid-fast bacilli (AFB) identification in tissue section are less because xylene and formalin affect the sensitivity of Ziehl-Neelsen (ZN) method to detect Mycobacterium tuberculosis in histopathology sections.[5]
Although formalin-induced autofluorescence and
acid-fast stain of CLCs are probably nonspecific phenomena, use of the fluorescent microscope and the Ziehl-Neelsen stain can successfully help in the diagnosis of acute-phase EC, especially in samples with massive tissue necrosis.
No
acid-fast bacilli were noted on Ziehl-Neelsen staining.
A novel method of staining
acid-fast bacilli in sputum containers.
In summary, the diagnosis of cutaneous tuberculosis is based on clinical features, demonstration of
acid-fast bacilli on smear, tissue culture, skin biopsy, and in recent years, PCR.
Applicants with chest radiographs suggestive of tuberculosis now are required to submit three sputum specimens for both sputum smears for
acid-fast microscopy and mycobacterial culture.
Smears stained with ZN stain (Figure 2:
Acid-fast bacilli in ZN stain, 100 X.) were examined under oil immersion objective for AFB.
leprae infection based on the clinical and histological features and skin smear
acid-fast staining.{Table 1}
In this nonrandom sample of 26 TB outbreaks in the United States during 2002-2011, we found that characteristics common among TB cases that started outbreaks included pulmonary TB smear-positive for
acid-fast bacilli, patient substance abuse, and prolonged infectious periods.
Disseminated histiocytic infiltration of either aggregates or sheets of epithelioid cells containing
acid-fast bacilli, in the absence of caseous necrosis, were observed in different organs of the infected doves, especially lungs (23/23), intestines (9/23).
staining was performed on unfixed slide after heat fixing for
acid-fast bacilli.
