Slit-skin smear from the earlobe did not reveal acid-fast
The chances of acid-fast
bacilli (AFB) identification in tissue section are less because xylene and formalin affect the sensitivity of Ziehl-Neelsen (ZN) method to detect Mycobacterium tuberculosis in histopathology sections.
Although formalin-induced autofluorescence and acid-fast
stain of CLCs are probably nonspecific phenomena, use of the fluorescent microscope and the Ziehl-Neelsen stain can successfully help in the diagnosis of acute-phase EC, especially in samples with massive tissue necrosis.
bacilli were noted on Ziehl-Neelsen staining.
A novel method of staining acid-fast
bacilli in sputum containers.
In summary, the diagnosis of cutaneous tuberculosis is based on clinical features, demonstration of acid-fast
bacilli on smear, tissue culture, skin biopsy, and in recent years, PCR.
Applicants with chest radiographs suggestive of tuberculosis now are required to submit three sputum specimens for both sputum smears for acid-fast
microscopy and mycobacterial culture.
Smears stained with ZN stain (Figure 2: Acid-fast
bacilli in ZN stain, 100 X.) were examined under oil immersion objective for AFB.
leprae infection based on the clinical and histological features and skin smear acid-fast
In this nonrandom sample of 26 TB outbreaks in the United States during 2002-2011, we found that characteristics common among TB cases that started outbreaks included pulmonary TB smear-positive for acid-fast
bacilli, patient substance abuse, and prolonged infectious periods.
Disseminated histiocytic infiltration of either aggregates or sheets of epithelioid cells containing acid-fast
bacilli, in the absence of caseous necrosis, were observed in different organs of the infected doves, especially lungs (23/23), intestines (9/23).
staining was performed on unfixed slide after heat fixing for acid-fast