acid elution

acid elution

The use of buffered HCl to remove (elute) antigens from the surface of red cells in order to identify those antigens capable of producing a haemolytic reaction or agglutination.
References in periodicals archive ?
Bound antibody can be recovered by acid elution (14).
A potential drawback of affinity purification is that the obtained DAT could be denatured by acid elution.
Acid elution antibody reagent for the determination of, test
The adsorbents are then removed from the water and the metals are readily extracted using simple acid elution methods.
Scientists then remove the adsorbents from the water and the metals are readily extracted using a simple acid elution method.
2) We found that the Rosette and acid elution techniques were effective at detecting a fetomaternal hemorrhage (FMH) requiring additional units of Rh immune globulin (Rhlg; sensitivity 1.
In our view, improving the effectiveness of RhIg dosing would require 2 things: (1) a more accurate calculation of maternal blood volume, such as Dr Ramsey's RhIg Dose Calculator2; and (2) replacement of acid elution FMH quantitation with flow cytometry in all cases in which additional Rh immune globulin doses are indicated by initial Rosette or acid elution test results.
Actually, the fetal cells are resistant to this acid elution and stain a bright pink with the method we use.
2]-MG affinity-chromatography acid elution pattern is shown in Fig.
2]-MG concentration was in fraction 3, indicating that the acid elution caused dissociation of the [[alpha].
1 mol/L acetic acid), wash reagent II (100 mL of ethyl acetate, 100 [micro]L of glacial acetic acid), acid elution reagent (140 mL of hexane, 60 mL of ethyl acetate, 200 [micro]L of glacial acetic acid), and basic elution reagent (98 mL of ethyl acetate, 2 mL of concentrated ammonium hydroxide).