The mRNAs encoding several yield-related enzymes including acetyl CoA carboxylase, glucosyltransferase, phosphate translocator, and lipoxygenase were reprogrammed by nucleotide solutions with concomitant changes in the accumulation of cellulosic biomass and vegetable oil in peanut [11, 12, 14].
The cDNA inserts that were used as probes were those homologous to rRNA, and to mRNAs encoding acetyl CoA carboxylase (ACC), lipoxygenase (LO), glucosyltransferase, inorganic phosphate translocator, nitrate reductase, and glycinamide ribonucleotide (GAR) synthetase/GAR transformylase.
Molecular biology of GDH-based biotechnology: Some of the GDH-synthesized RNAs that were used as probes were those homologous to mRNAs encoding glucosyltransferase, phosphate translocator ; acetyl CoA carboxylase (ACC), lipoxygenase (LO) ; probes #1 for nitrate reductase (NR) and probe #2 for glycinamide ribonucleotide transformylase/glycinamide ribonucleotide synthetases (GART/GARS) (Table 1).
2004) also indicated that reduced lipogenesis in the mammary gland of lactating mice was caused by reducing acetyl CoA carboxylase
activity and mRNA abundance of acetyl CoA carboxylase
, the critical enzyme in de novo fatty acid synthesis, and also inhibited mammary desaturation by reducing mammary stearoyl-CoA desaturase activity and mRNA abundance.