The specimens of blood for the sepsis work up were obtained from each neonate prior to the commencement of the antibiotics, which included hematological parameters like total leukocyte count (TLC), absolute neutrophil count
(ANC), immature/total neutrophil cell ratio(I/T ratio), microESR, blood culture and sensitivity, CRP and PCT estimation.
About 3-4ml blood was drawn using a sterile syringe, out of which 1ml of the blood sample was inoculated aseptically in to a blood culture bottle, 1ml of blood, was allowed to clot in a sterile bottle to collect serum for the estimation of C-reactive protein and the remaining 2ml of blood was collected in a sterile bottle containing the anticoagulant EDTA (2-2.5 mg/ml) for estimation of Total WBC count, Absolute neutrophil count
, Band cell count.
[greater than]4% LUCs should be reported only when commented as "atypical lymphs." Left Shift If + in combination with normal absolute neutrophil count
and normal cytogram, verify instrument results.
He suggested closer monitoring of patients below 500 neutrophils/[mm.sup.3] For example, if a patient with neutropenia and fever is afebrile by day 3 and has an absolute neutrophil count
(ANC) greater than 500 neutrophils/[mm.sup.3], one can stop antibiotics by day 7.
In a study of 256 children aged 3-35 months, the use of C-reactive protein (CRP) in addition to either WBC or absolute neutrophil count
slightly increased the sensitivity but also significantly decreased the specificity of the screening tests.
C-reactive protein concentration is superior to the absolute neutrophil count
and the white blood cell count in picking up clinically undetectable serious bacterial infection in febrile children requiring antibiotic therapy, said Dr.
Complete haematological remission is defined as absolute neutrophil count
(ANC) 1.0 x 109/L, platelet count 100,000/ul and bone marrow blast 20% blasts, Sudan black negativity of the blast cells and characteristic immunophenotype (for B-ALL >20% of Blast cells express tdt, CD10, CD 19, CD 22, cCD79a and for T-ALL, cCD 3, CD 5, CD 7).
The NLR was calculated by dividing absolute neutrophil count
by the absolute lymphocyte count; similarly, the PLR was calculated by dividing the absolute platelet count by the absolute lymphocyte count, and the NMR was calculated by dividing the absolute monocyte count by the absolute lymphocyte count.
Even though the complete triad is not indispensable for the diagnosis, persistent neutropenia with absolute neutrophil count
below 1500/mm3 is necessary  and predisposes to recurrent bacterial infection that can lead to septic shock.
Total leukocyte count, absolute neutrophil count
and platelet count did not show statistically significant correlation (Table-6).
Neutropenia is defined as an absolute neutrophil count
(ANC) of 500 cells/mcl less (BMJ, 2018).
Some measurement indicators (age, BMI, hemoglobin, and serum albumin) were converted into categorical variables based on clinically and commonly used cut-off values, and some measurement indicators (systolic blood pressure, diastolic blood pressure, absolute neutrophil count
, absolute lymphocyte count, creatine kinase-MB [CKMB], total blood cholesterol, blood glucose, blood calcium, blood phosphorus, pkt/v, rkt/v, and tkt/v) were converted into categorical variables based on quartiles.