In my 2004 report, I noted that a report published in Nature established succinate and a-ketoglutarate
as important signaling molecules.
The activities of hepatic mitochondrial enzymes such as isocitrate dehydrogenase (ICH) a-ketoglutarate dehydrogenase (KDH) succinate dehydrogenase (SH) malate dehydrogenase (MDH) NADPH dehydrogenase and cytochrome C oxidase were significantly decreased in CCl4- hepatotoxic rats and administration of corosolic acid brought back these parameters towards normal.
Tricarboxylic acid cycle enzymes analysis: The enzyme activity of isocitrate dehydrogenase was assayed by the method of Bell and Baron (1960) a calibration curve was established with a-ketoglutarate as standard.
The GDH elevation in all tissues (Table I) also suggests the possibility of a need for a-ketoglutarate in the Krebs cycle for the liberation of energy.
GDH catalyses the reversible reaction of oxidative deamination of glutamate to a-ketoglutarate and ammonia (Begum and Vijayaraghavan 1998) and plays an important role in the catabolism and biosynthesis of amino acids.
According to the detected genes for glucose metabolism, they elucidated the fungal carbohydrate metabolism pathway and postulated that the metabolites for Neocallimastix frontalis were acetate, ethanol, formate, lactate, succinate and a-ketoglutarate
, which was oxidized from oxaloacetate by aconitase and isocitrate dehydrogenase.
From the a-ketoglutarate
languishing in the melting pot,
Thiamine and its dependent enzymes, transketolase (TK), pyruvate dehydrogenase, a-ketoglutarate
dehydrogenase and the branched chain a-ketoglutarate
dehydrogenase complex are involved in the maintenance of NADPH levels and carbohydrate metabolism in the cell8.
3) catalyses the reversible oxidative deamination of glutamate (glu) to a-ketoglutarate ([alpha]KG).
14) catalyses the formation of glutamate from glutamine and a-ketoglutarate.