polymerase

[pah-lim´er-ās]

an enzyme that catalyzes polymerization.

polymerase chain reaction a rapid technique for in vitro amplification of specific DNA or RNA sequences, allowing small quantities of short sequences to be analyzed without cloning.

pol·ym·er·ase

(pol-im'ĕr-ās),

General term for any enzyme catalyzing a polymerization, as of nucleotides to polynucleotides, thus belonging to EC class 2, the transferases.

Farlex Partner Medical Dictionary © Farlex 2012

polymerase

(pə-lĭm′ə-rās′, -rāz′, pŏl′ə-mə-)

Any of various enzymes, such as DNA polymerase, RNA polymerase, or reverse transcriptase, that catalyze the formation of polynucleotides of DNA or RNA using an existing strand of DNA or RNA as a template.

POLA1

A gene on chromosome Xp22.1-p21.3 that encodes the catalytic subunit of DNA polymerase that plays a key role in initiating DNA replication.

pol·ym·er·ase

(pŏ-lim'ĕr-ās)

General term for any enzyme catalyzing a polymerization, as of nucleotides to polynucleotides, thus belonging to EC class 2, the transferases.

Medical Dictionary for the Health Professions and Nursing © Farlex 2012

polymerase

Any enzyme that promotes the linkage of a number of similar or identical chemical subunits into repetitive long-chain molecules (polymers), especially of NUCLEOTIDES to form DNA or RNA. Derivation as in POLYMER with the -ase suffix denoting an enzyme.

Collins Dictionary of Medicine © Robert M. Youngson 2004, 2005

polymerase

an enzyme that catalyses the joining of DNA or RNA nucleotides.

Collins Dictionary of Biology, 3rd ed. © W. G. Hale, V. A. Saunders, J. P. Margham 2005

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References in periodicals archive

The structural coordinates for human DNA polymerase [alpha] in ternary complex with RNA-primed DNA template and aphidicolin were obtained from the PDB-ID: 4Q5V with 2.50 A resolutions.

Inhibitory Mechanism Exhibited by Phenol-based Natural Products against DNA Polymerase [alpha] from Psoralea corylifolia by Molecular Docking

Reactions that reached exhaustion within 30 min were calibrated by normalizing the minimum fluorescent value of each progress-curve to zero, and the maximum fluorescent value to the average number of extendable bases per polymerase. Then, the initial slope of the curve corresponds to the average initial rate in nucleotides per second polymerase (nt/s/poly).

The Effect of Single Mismatches on Primer Extension

Based on the 2.5 kb gene sequence information of Taq DNA polymerase gene (Di et al., 2012), a pair of primers (Taq1: 5'-GGCCGAATTCATGCTGCCCCTCTTTGAGCCC-3') and Taq2: 5'-GGCCGAATTCTCACTCCTTGGCGGAGAGCC-3') were designed, with EcoRI located at each end of the gene.

Development of Plant Expression Vector with Taq DNA Polymerase Gene to Yield Heat-tolerant Maize Lines

Several polymerase chain reaction (PCR)-based techniques have been developed for routine clinical malaria diagnosis, e.g.

A PCR method based on 18S rRNA gene for detection of malaria parasite in Balochistan

This study has confirmed the existence of HBV DNA polymerase resistance mutations for NAs in 24 (8.9%) of 269 treatment-naive patients, composed of five primary drug resistance mutations (rtT184G, rtS202I, rtM204I/V, rtN236T, and rtM250V), one secondary resistance mutation (rtL180M), and one codon mutation that might be baseline polymorphisms without any clinical significance (rtV207I).

Prevalence of mutations in HBV DNA polymerase gene associated with nucleos(t)ide resistance in treatment-naive patients with Chronic Hepatitis B in Central China

As it can be seen from the results of electrophoresis, Taq polymerase is capable to effectively incorporate in the sequence of a strand only modified nucleotide 5-(C8 Alkyne)-2'-Deoxyuridine-5'-Triphosphate.

Systematic comparison of the capability of modified nucleoside triphosphates to act as a substrate for thermostable DNA polymerases during PCR

Previous studies have shown that PB1-F2 of the A/WSN/33 strain (WSN;H1N1) upregulates viral polymerase activity in vitro [11].

PB1-F2 Amino Acids Regulate Influenza A Viral Polymerase Activity

Therefore, the methods for rescuing virus in vivo with high efficiency and no need for RNA preparation in vitro are urgently established.T7 RNAP, one of single-subunit RNA polymerases, is widely employed to generate high specific activity labeled RNA probes, RNA for in vitro translation, biologically active mRNA, and preparative quantities of defined length RNA by the run off transcription method (Schenborn and Mierendorf, 1985).

Stable Expression of T7 RNA Polymerase Mediated by Lentivirus in MDBK Cells

In vivo DNA replication methods for most organisms rely on a class of enzymes known as DNA helicases, which act to locally unwind dsDNA to create small ssDNA regions suitable for polymerase activity.

Alternative nucleic amplification strategies, part 2: LCA and HDA

One could suppose that stabilization of the beta-structured state of PB1 N-terminus would result in inhibition of the interaction of this protein with PA, polymerase complex assembly, and reproduction of viral RNA.

Structural features of the peptide homologous to 6-25 fragment of influenza a PB1 protein

By direct sequencing of clones from two independent PCR experiments with Taq polymerase, we observed 99 unique mutations out of >100 kbp of target DNA sequence.

Error rate comparison during polymerase chain reaction by DNA polymerase

Polymerase Assay for Incorporation opposite 5' -Neighboring Bases of G or Oz.

Effects of stability of base pairs containing an oxazolone on DNA elongation