The detected parasites were fixed with alcohol-formalin-acetic acid (AFA), dyed with Mayer's
carmalum, and mounted with Canada balsam, according to the study by Pritchard and Kruse (8).
Eyes were extracted and the parasites were removed from the vitreous humor, counted, fixed and, then, identification plates were prepared with Mayer's
carmalum staining according to EIRAS et al.
solution (a mixture of ethyl alcohol 92 ml + formalin 5 ml + acetic acid 3 ml) for 25 hours stained with Mayer's
carmalum dehydrated in graded series of alcohols cleared in clove oil and xylene and mounted permanently on glass slides in Canada balsam by the usual procedure.
Trematode specimens recovered from these fishes were fixed in AFA solution under slight cover glass pressure for 24 hours, washed several times with 70% alcohol, stained with Mayer's
carmalum, dehydrated in graded series of alcohols, cleared in clove oil and xylene and mounted permanently in Canada balsam.
Specimens were fixed in AFA solution, a mixture of 70% ethyl alcohol, formalin and acetic acid in the ratio of 90:7:3, under slight cover glass pressure for 24 hours, washed several times with 70% ethyl alcohol, stained with Mayer's
Carmalum, dehydrated in graded series of alcohols, cleared in clove oil and xylene and mounted permanently in Canada balsam.
solution under slight glass slide pressure, stained with Mayer's
carmalum, dehydrated in graded series of alcohol cleared in clove oil and xylol, and mounted permanently in Canada balsam.
These were fixed in hot 70% ethyl alcohol, stained with Mayer's
carmalum, dehydrated in graded series of alcohols, cleared in clove oil and xylene and mounted permanently in Canada balsam.