NTHi strains were selected with the aim of including isolates of all known genetic clades (19).
Another group of investigators reported that a thing called "phasevarion," which is fancy lingo for an on-off switch is at the root of more virulence in
ntHi. (3) It seems that some strains of
ntHi have a version of the ModA2 gene, which is always turned off, while other strains have a gene that is always on.
Following
NTHi stimulation, cells were fixed with 4% paraformaldehyde, followed by incubation with primary antibodies at 1: 100-1: 400 dilutions.
Results: The results demonstrated that hmwA was detected in 61% of
NTHi isolates.
However,
NTHI bacteria build biofilms, sticky protective covers that evade antibiotics and allow the bacteria to flourish in the middle ear, nasal passages and lungs to cause repeat infections.
NTHI has so far trained 700 Saudis since its inception in May last year.
In that case, is the persistence of
NTHi in the effusion explained only by the fact that
NTHi is protected by a phenotypic switch between a biofilm state and a free-floating (planktonic) state?
tonB-specific sequences were amplified from
NtHi 2019,
NtHi 3198, H parainfluenzae 4201, and H parainfluenzae 4190 using primers HitonBF and HitonBR.
influenzae (
NTHi) vaccine for prevention of otitis media