After 14 days of cultivation, chondrogenic pellets were stained with
Alcian Blue Stain.
Inset, fungal capsule exhibits marked
Alcian Blue stain positivity.
Slides were stained with hematoxylin and eosin and
alcian blue stain. Osteocytes were counted in a defined unit area under high power in the two groups and the average of the two groups was compared.
Caption: FIGURE 3:
Alcian blue stain showing increased dermal mucin deposition (Alcian blue; original magnification x10).
Rehydrated tissue sections were immersed in
Alcian blue stain solution (Alcian blue 8GX, 1 gm / 100 mL 3% acetic acid pH 2.5, Sigma-Aldrich, St.
It stains with
alcian blue stain at pH 2.5 and is susceptible to hyaluronidase digestion.
Histopathological examination using the
Alcian blue stain revealed mucin deposition in the reticular dermis with connective tissue separation [Figure 1]d.
(b) Intracellular and extracellular mucin deposition (focal distribution) is present (
Alcian blue stain, original magnification x400).
After staining, extracellular matrix was taken to dark blue stain where the so-called
Alcian blue stain had metachromatic appearance to extracellular matrix (Figure 5(a)).
(a) A palisading granuloma with central collagen degeneration (H&E stain, magnification x100); (b)
Alcian blue stain showing increased mucin deposition within the granuloma (
Alcian blue stain, magnification x100); (c) several annular red-brown plaques involving the thigh.
Other stains can be applied according to the need of diagnosis, for example, Gram's stain, Ziehl-Neelsen stain, Periodic acid-Schiff stain,
Alcian blue stain, and Papanicolaou stain.
Histological cutaneous examination revealed epidermal atrophy, and superficial and deep perivascular and perifollicular dermatitis with focally increased dermal mucin highlighted by
Alcian blue stain, suggesting lupus erythematosus (LE).