SDS-PAGE (a) and Native-PAGE (b) zymography
of purified trypsin from hepatopancreas of Dosidicus gigas.
The activity of MMP-2 and 9 were detected in zymography
On the other hand, the zymography
method proposed by Platt, Randall and Hanjoong  for cathepsin detection was used.
technique with gelatin as a substrate indicated that both the serum and the synovial fluid of all investigated patients contain matrix metalloproteinase 2 and 9 (Fig.
a) An equal amount (50 [micro]g) of conditioned culture media from each sample was subjected to gelatin zymography
Determination of MMP-9 Activity by Gelatin Zymography
The advantage of zymography
is that it identifies individual proteins in a mixture of extracted enzymes and gives a rapid estimation of enzyme molecular weights (Lacks and Springhorn 1980).
Distribution of microbial-and root-derived phosphatase activities in the rhizosphere depending on P availability and C allocation-Coupling soil zymography
with 14C imaging.
Samples for electrophoresis and zymography
were dissolved in 2X Laemmli Sample Buffer (65.
Western analysis was used to validate minimal cross-reactivity in the chip, and zymography
was used to validate MMP presence in urine from cancer patients.
Different pattern of zymography
between human gynecologic normal and malignant tissues.
Detection of TIMP-2-like protein in Atlantic cod (Gadus morhua) muscle using two-dimensional real-time reverse zymography