To conduct histological analysis, mice ( n = 7 in each group) were anesthetized with 30 mg/kg of Zoletil
50 (Virbac, Carros, France) and perfused by the aorta with 4% paraformaldehyde in 0.
02 mg/kg of atropine sulfate (Laboratorios Erma, Chile) and then anaesthetized by intramuscular injection of 10 mg/kg of Zoletil
50 (Zolazepam-Tiletamine hydrochloride) (Tebet et al.
50 was purchased from Provisions Marketing, Quezon City.
3mg/kg) (Labesfal) and anesthetized by intramuscular injection with Zoletil
anesthetic (20-30mg/kg) (Virbac).
Neuroleptanalgesia with xylazine and zoletil
was applied to the experimental animals.
02 mg/kg and zoletil
(R), Virbac, Carros, France) at 1 mg/kg.
Defect of 3x4-mm extension was modeled in the proximal third of tibial shaft of animals by milling at low speed under general anesthesia (Rometar8 mg/kg and Zoletil
4 mg/kg IM).
In addition, anesthesia was induced by injecting the pigs with Strenial (Janssen Pharmaceutica, Belgium) and Zoletil
50 (Virbac Lab, Korea) before surgery.
Before treadmill running habituation, the rats were anesthetized with zoletil
50 (10 mg/kg, i.
70 ml of Zoletil
50 (Tiletamine), so that the monkey could be taken for a functional MRI study.
Immobilization of walrus with etorphine hydrochloride and Zoletil
(Virbac Laboratories, Carros, France) was administered in a solution of 200 mg/mL, at a dose of 5-10 mg/kg body mass (BM).