Ziehl-Neelsen stain

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Ziehl-Neel·sen stain

(zēl nāl'sen),
a method for staining acid-fast bacteria using Ziehl stain, decolorizing in acid alcohol, and counterstaining with methylene blue; acid-fast organisms appear red, other tissue elements light blue; a modification of this stain is also used for Actinomycetes and Brucella.

Neelsen,

Friedrich K.A., German pathologist, 1854-1894.
Ziehl-Neelsen stain - see under Ziehl

Ziehl,

Franz, German bacteriologist, 1857-1926.
Ziehl stain - a carbol-fuchsin solution of phenol and basic fuchsin used to demonstrate bacteria and cell nuclei.
Ziehl-Neelsen stain - a method for staining acid-fast bacteria.
References in periodicals archive ?
Such extension of the infiltrate might compromise the deep dermis until the hypodermis, thus preventing macrophages from eliminating the bacilli and allowing them instead to accumulate in the cytoplasm, sometimes in globies as demonstrated by Ziehl-Neelsen staining (Figure 1).
Laboratory tests (direct smear examination after Ziehl-Neelsen staining, culture, and IS2404 PCR) were negative.
Results: Out of 480 samples, 06 (2.1%) were found positive for M.TB through Ziehl-Neelsen staining while 10 (2.8%) were positive through LJ and BACTEC MGIT-960 culture.
Giemsa and Ziehl-Neelsen staining were negative for fungi and acid-fast bacilli, respectively.
(d) Ziehl-Neelsen Staining showing scant AFB [ZN Staining (1000x)].
Mycobacterium tuberculosis detection staining technique by fluorescence microscopy is about 10% more sensitive than the Ziehl-Neelsen staining technique and has a similar specificity.
Color and odor of the fluid, glucose, protein lactate dehydrogenase, Gram staining, Ziehl-Neelsen staining, cytology and cytopathology of the fluids were mandatory investigations.
Acid-resistant bacterium was observed with Ziehl-Neelsen staining in microbiologic examination of the samples obtained.
The Ziehl-Neelsen staining showed bacilli forming globi.
In the case of tuberculosis infection, demonstration of the mycobacteria can be done with Ziehl-Neelsen staining or by immunofluorescence using auramine-rhodamine.