Such extension of the infiltrate might compromise the deep dermis until the hypodermis, thus preventing macrophages from eliminating the bacilli and allowing them instead to accumulate in the cytoplasm, sometimes in globies as demonstrated by
Ziehl-Neelsen staining (Figure 1).
Laboratory tests (direct smear examination after
Ziehl-Neelsen staining, culture, and IS2404 PCR) were negative.
Results: Out of 480 samples, 06 (2.1%) were found positive for M.TB through
Ziehl-Neelsen staining while 10 (2.8%) were positive through LJ and BACTEC MGIT-960 culture.
Giemsa and
Ziehl-Neelsen staining were negative for fungi and acid-fast bacilli, respectively.
(d)
Ziehl-Neelsen Staining showing scant AFB [ZN Staining (1000x)].
Ziehl-Neelsen staining was performed on smears of 31 biopsy specimens.
Mycobacterium tuberculosis detection staining technique by fluorescence microscopy is about 10% more sensitive than the
Ziehl-Neelsen staining technique and has a similar specificity.
Color and odor of the fluid, glucose, protein lactate dehydrogenase, Gram staining,
Ziehl-Neelsen staining, cytology and cytopathology of the fluids were mandatory investigations.
Acid-resistant bacterium was observed with
Ziehl-Neelsen staining in microbiologic examination of the samples obtained.
The
Ziehl-Neelsen staining showed bacilli forming globi.
In the case of tuberculosis infection, demonstration of the mycobacteria can be done with
Ziehl-Neelsen staining or by immunofluorescence using auramine-rhodamine.
