Ziehl-Neelsen stain


Also found in: Dictionary, Thesaurus, Encyclopedia, Wikipedia.

Ziehl-Neel·sen stain

(zēl nāl'sen),
a method for staining acid-fast bacteria using Ziehl stain, decolorizing in acid alcohol, and counterstaining with methylene blue; acid-fast organisms appear red, other tissue elements light blue; a modification of this stain is also used for Actinomycetes and Brucella.

Neelsen,

Friedrich K.A., German pathologist, 1854-1894.
Ziehl-Neelsen stain - see under Ziehl

Ziehl,

Franz, German bacteriologist, 1857-1926.
Ziehl stain - a carbol-fuchsin solution of phenol and basic fuchsin used to demonstrate bacteria and cell nuclei.
Ziehl-Neelsen stain - a method for staining acid-fast bacteria.
References in periodicals archive ?
With regard to smear, a positive Ziehl-Neelsen stain was considered as a potential BU case.
The FNAB tests showed numerous lymphocytes and histiocytes with emperipolesis (Figure 3, B); Ziehl-Neelsen stain results for acid-fast bacilli were negative, and the features were considered most consistent with Rosai-Dorfman disease.
The histopathology of the tissue obtained by surgery revealed the existence of granulomas with Gram, Giemsa and Ziehl-Neelsen stains negative.
Sputum smear stained by Ziehl-Neelsen stain was 2+ positive for acid-fast bacilli (AFB).
The Ziehl-Neelsen stain of the tissue did not reveal any acid-fast bacilli.
Moreover, we performed phosphotungstic acid-hematoxylin, Ziehl-Neelsen stain, periodic acid-Schiff reaction, periodic acid-Schiff diastase reaction, and Grocott histochemical stains.
Duodenal biopsy from the area of the mass demonstrated two poorly formed epitheloid granulomas within the lamina propria, with a Ziehl-Neelsen stain highlighting several acid-fast bacilli.
Histological analysis revealed necrotising caseating granulomatous inflammation, and the Ziehl-Neelsen stain was positive for acid-fast bacilli.
Ziehl-Neelsen stain was done in clinically suspected tuberculosis cases.
KEY WORDS: Tuberculosis, lymphadenopathy, fine-needle aspiration cytology, Ziehl-Neelsen stain