Ziehl-Neelsen method


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Related to Ziehl-Neelsen method: Acid fast

Ziehl-Neelsen method

(zēl-nēl′sĕn)
[Franz Ziehl, Ger. bacteriologist, 1857–1926; Friedrich Karl Adolf Neelsen, Ger. pathologist, 1854–1894]
A method for staining Mycobacterium tuberculosis. A solution of carbolfuchsin is applied, which the organism retains after rinsing with acid alcohol
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Out of these 23 cases, AFB were demonstrated in 20 cases by Auramine-O method and in 13 cases by Ziehl-Neelsen method.
The sputum smears were prepared, stained and examined by the Ziehl-Neelsen method according to WHO/IUATLD guidelines and at least 100 microscopic fields using 100X oil immersion lens were examined to declare a slide negative.
Sensitivity of Ziehl-Neelsen method for centrifuged deposit smears of sputum samples transported in cetyl-pyridinium chloride.
Screening usually includes questions about TB symptoms, testing of sputum specimens by smear microscopy (using acid-fast--bacilli staining by the Ziehl-Neelsen method), and chest radiography (depending on availability).
Oocysts of Cryptosporidium species were identified using smears stained with the modified Ziehl-Neelsen method following concentration by the formol ether oocysts concentration technique as described by Henriksen and Pohlenz (1981).