A direct calibration graph was constructed for ZIC1 and ZIC5 columns and it was found that the linear range (10-1000 ng.ml-1), RSD% (0.41-1.55), LOD (1.55 and 2.56 ng.ml-1), LOQ (5.17 and 8.53 ng.ml-1), Recovery% (101.06 +- 1.15 and 100.47 +- 0.12) and Erel% (1.666 +- 0.78 and 0.47 +- 0.12), respectively.
The second goal of our work is to investigate the influence of chain length between the charges (one methylene group ZIC1 and five methylene groups ZIC5).
The stationary phases (ZIC1 and ZIC5) used for the RANH separation were a self-made via grafted sulfobetaine monomers (H2C=CHC6H4CH2N+ (CH3)2-CH2-SO3-and H2C=CHC6H4CH2N+(CH3)2-(CH2)5-SO3) [10, 11, 13, 16-18] onto the PS/DVB using PEEK columns (100 mm x 4 mm I.D.).
The ZIC1 and ZIC5 columns have capacity 432 and 488 ueq g-1 , respectively.
It should be noted that retention of RANH in ZIC5 column exhibits the highest retention in comparison with the behavior of RANH in ZIC1 column.
RANH exhibits hydrophobic (RP) behavior for both ZIC1 and ZIC5 columns (Fig.
It is worth to be mentioned that the retention of RANH shows the highest retention in ZIC5 columns is due to the space length between charged groups.
RANH with an isoelectric point 13.24, the retention time decreased on ZIC1 and ZIC5 columns due to the protonation of the amino group in RANH.
A calibration graph of RANH established by plotting the area versus concentration of RANH and shows the range concentration (10-1000 ng mL-1) of ZIC1 and ZIC5 columns (Fig.