A direct calibration graph was constructed for ZIC1 and ZIC5 columns and it was found that the linear range (10-1000 ng.ml-1), RSD% (0.41-1.55), LOD (1.55 and 2.56 ng.ml-1), LOQ (5.17 and 8.53 ng.ml-1), Recovery% (101.06 +- 1.15 and 100.47 +- 0.12) and Erel% (1.666 +- 0.78 and 0.47 +- 0.12), respectively.
The second goal of our work is to investigate the influence of chain length between the charges (one methylene group ZIC1 and five methylene groups ZIC5).
The stationary phases (ZIC1 and ZIC5) used for the RANH separation were a self-made via grafted sulfobetaine monomers (H2C=CHC6H4CH2N+ (CH3)2-CH2-SO3-and H2C=CHC6H4CH2N+(CH3)2-(CH2)5-SO3) [10, 11, 13, 16-18] onto the PS/DVB using PEEK columns (100 mm x 4 mm I.D.).
The ZIC1 and ZIC5 columns have capacity 432 and 488 ueq g-1 , respectively.
It should be noted that retention of RANH in ZIC5 column exhibits the highest retention in comparison with the behavior of RANH in ZIC1 column.
RANH exhibits hydrophobic (RP) behavior for both ZIC1 and ZIC5 columns (Fig.
Transcription Adjusted Motif ID factor p value p value WT1_MOUSE.H11MO.1.A Wt1 8.014E-06 0.004246 EGR2_MOUSE.H11MO.0.A Egr2 5.517E-05 0.02887 ZN281_MOUSE.H11MO.0.A Zn281 5.737E-05 0.03001 ZIC1_MOUSE.H11MO.0.B Zic1
7.868E-05 0.04092 SP5_MOUSE.H11MO.1.C Sp5 9.138E-05 0.04737 Figure 1: Global expression patterns of genes at the nine time points of osteoblast differentiation.
, a combination of small molecules A-8301 (inhibitor of TGF-beta), thiazovivin (ROCK inhibitor), purmorphamine (SHH agonist), and valproic acid (VPA: inhibitor of histone deacetylase (HDAC)) was chosen, resulting in the production of NSC positive for Sox2, Pax6, PLZF, Dach1, Fam70a, Nr2f1, and Zic1
from murine fibroblasts, which were then differentiated into functional neurons, astrocytes, and oligodendrocytes positive for Tuj1, GFAP, and Olig2, respectively.
Cheng et al., "ZIC1
is silenced and has tumor suppressor function in malignant pleural mesothelioma," Journal of Thoracic Oncology, vol.
Schijvenaars et al., "Genetic variants in ZIC1
, ZIC2, and ZIC3 are not major risk factors for neural tube defects in humans," American Journal of Medical Genetics, vol.
We did not find any signal for the gene products of the genes family member 1, proteolipid protein 1, neuronatin, or olfactomedin 1 (ZIC1
, PLP1, NNAT, or OLFM1) with the antibodies described in Table 3.
Additionally, biomarkers of different molecular classes like proteins (e.g., mesothelin  and calretinin ) or methylated DNA (e.g., ZIC1
 and RASSF1A ) could be tested in combination with the miRNAs and might be added to a future marker panel in order to further improve the diagnostic performance.