In addition to SLUG, other transcription factors, such as SNAIL, TWIST, ZEB1, and ZEB2
, may inhibit ECAD transcription and contribute to epithelial-mesenchymal transition (33).
54) Indeed, cancer cells transfected with mir-200c demonstrated decreased expression of the mesenchymal markers ZEBI, ZEB2
, SNAIL, SLUG, increased expression of the epithelial marker E-cadherin, and decreased invasive capacity of the cells.
The miR-200 family regulates TGF-[sz]1-induced renal tubular epithelial to mesenchymal transition through Smad pathway by targeting ZEB1 and ZEB2
Since the loss of E-cadherin expression is considered a crucial event in EMT, EMT-TFs have been classified based on their ability to repress E-cadherin directly (SNAIL, SLUG, ZEB1, ZEB2
miRNAs Blanco asociado Funcion del miRNA en metastasis de tumor miR-10b HOXD10 Promotor miR-21 PTEN, PDCD4, TPM1, Maspin Promotor miR-373 CD44, LATS2 Promotor miR-520c CD44 Promotor miR-155 RhoA Promotor Let-7 Ras, HMGA2 Represor miR-335 SOX4, TNC Represor miR-126 Crk, IRS-1 Represor miR-146a ROCK1 Represor miR-101 EZH2 Represor Familia miR-200 ZEB1, ZEB2
determinaron que las mutaciones en el gen ZEB2 (Zinc finger E-boxbinding homeobox 2) (4), eran las responsables de esta enfermedad dada la importancia de esta proteina durante el proceso de embriogenesis (5).
El gen ZEB2 es responsable del SMW, confirmando el diagnostico, Para complementar el estudio y con la finalidad de realizar asesoria a los padres se realizo estudio de FISH (Hibridacion In situ con fluorescencia) para la zona cromosomica comprometida, los cuales fueron normales, por lo que se determino que el evento de microdelecion en nuestra paciente ocurrio de novo.
The miR-200 family determines the epithelial phenotype of cancer cells by targeting the E-cadherin repressors ZEB1 and ZEB2
siRNA knockdown of ZEB1 and ZEB2
impaired As-transformed p53lowHBEC migration and invasion.
Just messing with levels of a messenger RNA from another gene known as ZEB2
throws off PTEN protein levels and can lead to melanoma in mice, Pandolfi's group reported in another paper in the Oct.
Using pregnant mice as well as human uterine tissue, the researchers have uncovered a feedback cycle involving microRNAs, proteins called ZEB1 and ZEB2
, and the pregnancy-maintaining hormone progesterone, as well as genes and other factors that control contraction of the uterus.
Specifically, these authors indicate that all the latency type I genes such as Bam HI-A rightward reading frame (BARF0), EBERs, EBNA1, and LMP2A have a synergetic effect on these processes and contribute to the downregulation of the mature miR-200 family and the subsequent upregulation of the E-cadherin transcription repressors, zinc finger E-box binding factor 1 (ZEB1) and ZEB2
, resulting in the inhibition of E-cadherin expression and induction of the epithelial-to-mesenchymal transition (EMT) .