radioimmunoassay(redirected from Yalow-Berson method)
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a sensitive assay method that can be used for the measurement of minute quantities of specific antibodies or any antigen, such as a hormone or drug, against which specific antibodies can be raised. An assay for a specific hormone uses antihormone antibody produced by injecting the human hormone into an animal, such as a rabbit, and hormone that has been labeled with a radioisotope. These are mixed with the assay specimen and the antigen (hormone) bound to antibody is separated from the unbound antigen by chromatography or other means. Because any hormone in the assay specimen competes with the radiolabeled hormone for antibody binding sites, the amount of hormone in the specimen is inversely proportional to the radioactivity of the bound fraction or directly proportional to the activity of the free fraction. This is the standard method for clinical laboratory measurements of hormones and is also used for therapeutic drug monitoring, drug abuse screening, and other laboratory tests.
Miller-Keane Encyclopedia and Dictionary of Medicine, Nursing, and Allied Health, Seventh Edition. © 2003 by Saunders, an imprint of Elsevier, Inc. All rights reserved.
An immunologic (immunochemical) procedure that uses competitive inhibition between a radioisotope-labeled antigen or other substance and unlabeled antigen for antiserums, binding as a means to quantify the agent of interest; any method for detecting or quantitating antigens or antibodies using radiolabeled reactants. Minute quantities of enzymes, hormones, or other substances can be assayed.
Farlex Partner Medical Dictionary © Farlex 2012
n. Abbr. RIA
A technique used to quantitate minute amounts of a substance, such as a hormone, in blood or another liquid. It involves the use of radioisotopes and of antibodies to the substance being investigated.
The American Heritage® Medical Dictionary Copyright © 2007, 2004 by Houghton Mifflin Company. Published by Houghton Mifflin Company. All rights reserved.
McGraw-Hill Concise Dictionary of Modern Medicine. © 2002 by The McGraw-Hill Companies, Inc.
An immunologic (immunochemical) procedure that uses the competition between radioisotope-labeled antigen (hormone) or other substance and unlabeled antigen for antiserums, resulting in quantitation of the unlabeled antigen; any method for detecting or quantitating antigens or antibodies using radiolabeled reactants.
Medical Dictionary for the Health Professions and Nursing © Farlex 2012
radioimmunoassayAny method of measuring the extent of linkage between ANTIGEN and ANTIBODY in which one or other of these is labelled with a radioactive substance (radionuclide). Measurement of radiation can be remarkably precise.
Collins Dictionary of Medicine © Robert M. Youngson 2004, 2005