Shotgun Sequencing

(redirected from Whole genome shotgun)
Also found in: Acronyms.
The sequencing of a large DNA segment by sequencing of randomly-derived subsegments, whose order and orientation within the large segment are unknown until the final assembly of overlapping sequences.
References in periodicals archive ?
Whitehead generated 5kb whole genome shotgun libraries that Agencourt used to generate the sequence data.
This whole genome shotgun project has been deposited at DDBJ/EMBL/Genbank under the accession LCVM00000000.