Shotgun Sequencing

(redirected from Whole genome shotgun)
Also found in: Acronyms.
The sequencing of a large DNA segment by sequencing of randomly-derived subsegments, whose order and orientation within the large segment are unknown until the final assembly of overlapping sequences.
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References in periodicals archive ?
The complete map will be useful in examining the unique sequences to be used for possible breed differentiation and to enhance the Whole Genome Shotgun data compilation.
In the May issue of Nature Biotechnology, scientists report on the sequencing of the 30-million base-pair genome of Phanerochaete chrysosporium strain RP78 using a whole genome shotgun approach.
In conjunction with the significant preliminary mapping and sequencing progress contributed by Jean Weissenbach's group at GenoScope in Envry, France, Agencourt provided another 2 million double-ended whole genome shotgun sequencing reads required to unveil the mystery of this genome.
Whitehead generated 5kb whole genome shotgun libraries that Agencourt used to generate the sequence data.
com), a business unit of Venter's Celera Genomics, introduced its GenomeAssembler, a package for all types of sequence assembly including whole genome shotgun projects.
Hunkappiller, president of Applied Biosystems, was honored for his contribution to the development of the automated high-throughput DNA sequencers and Venter, founder of Celera Genomics, for development of the whole genome shotgun strategy, the foundation said.
Venter left the NIH in 1992 to start his own company, using the whole genome shotgun sequence method--a method sharply criticized by many scientists at the time.
Whole Genome Shotgun (WGS) of haloarchaeon 3A1-DGR is an isolate from a salt crystallizer pond of Little Rann of Kutch, India and has been described recently (8) with accession number ATCR 00000000.
This whole genome shotgun project has been deposited at DDBJ/EMBL/Genbank under the accession LCVM00000000.SUBID: SUB939235.