Whole Genome Shotgun Sequencing

(redirected from Whole Genome Shotgun Method)
A semi-automated sequencing technique for determining the entire sequence of a particular organism, in which high-molecular-weight DNA is sheared into random fragments, size selected—usually 2, 10, 50, and 150 kb—and cloned into an appropriate vector
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The draft sequencing and assembly of the Fugu genome, announced last October, marked the first publicly released animal genome after the human sequence, and the first vertebrate genome publicly sequenced and assembled using the whole genome shotgun method.