Western blot

(redirected from Western blottings)
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An assay that detects specific proteins within a protein mixture by a multistep process consisting of electrophoresis on a slab gel, transfer of the proteins on the gel to a membrane followed by identification of the specific proteins by antibody staining
Segen's Medical Dictionary. © 2012 Farlex, Inc. All rights reserved.

Western blot

Immunoblot Molecular diagnostics An assay that IDs antibodies to proteins of specific molecular weights, used to confirm HIV infection–after a positive ELISA screening assay, Lyme disease. See Blot, Electrophoresis, Protein. Cf Northern blot, Southern blot.
McGraw-Hill Concise Dictionary of Modern Medicine. © 2002 by The McGraw-Hill Companies, Inc.

West·ern blot a·nal·y·sis

(wes'tĕrn blot ă-nal'i-sis)
A procedure in which proteins separated by electrophoresis in polyacrylamide gels are transferred (blotted) onto nitrocellulose or nylon membranes and identified by specific complexing with antibodies that are either pre- or post-tagged with a labeled secondary protein.
See also: immunoblot
Synonym(s): Western blot, Western blotting.
[coined to distinguish it from eponymic Southern blot a.]
Medical Dictionary for the Health Professions and Nursing © Farlex 2012

Western blot

A technique developed in 1979 that is used to confirm ELISA results. HIV antigen is purified by electrophoresis and attached by blotting to a nylon or nitrocellulose filter. The patient's serum is reacted against the filter, followed by treatment with developing chemicals that allow HIV antibody to show up as a colored patch or blot. If the patient is HIV-positive, there will be stripes at specific locations for two or more viral proteins. A negative result is blank.
Mentioned in: AIDS Tests
Gale Encyclopedia of Medicine. Copyright 2008 The Gale Group, Inc. All rights reserved.