Western blotting


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Western blotting

A method of detecting very small quantities of a protein of interest in a cell or body fluid. The sample is spread by ELECTROPHORESIS on a block of polyacrylamide gel. The separated proteins are then transferred, by blotting, to a thin plastic sheet to make them more accessible for reaction with an antibody specific for the protein of interest. The antibody-antigen complex can then be detected by rinsing the sheet with a second, radioactive, antibody that recognises the first. The sheet will now produce a dark band on X-ray film. See also SOUTHERN BLOTTING.

Western transfer

or

Western blotting

a method for transferring proteins, that have been separated by GEL ELECTROPHORESIS, from the gel to a membrane support. The method is named by analogy with SOUTHERN TRANSFER.
Western blot
Western blot analysis,
, Western blotting (wes'tĕrn blot, blot'ing)
Synonym(s): Western blot analysis.
References in periodicals archive ?
Use of western blotting for HIV testing is expected to increase in this region owing to the implementation of this program.
However, it was reported in 1998, that cTnT may also be degraded in the serum of AMI patients (20), which was subsequently confirmed by Western blotting in 2000 (21).
To compare and confirm the data generated in the phospho-flow cytometry assay, the phospho-flow technique was compared with Western blotting analysis of phosphorylation of p70S6K in healthy subject ( n = 1) and liver transplant patients receiving sirolimus, tacrolimus or cyclosporine ( n = 1 each) [Figure 1]a.
Also, TPN's wider linear dynamic range exhibits better linearity at the lower, more relevant protein levels (10 to 50 [micro]g) used in western blotting. The TPN technique is so well matched to this need that the target protein is directly normalized against the total protein concentration in each lane, making this technique more universal and accurate than HKP normalized experiments.
The expression levels and activity increases of caspase-3 by RT-PCR and Western blotting (Figure 2) raise the possibility of cardiomyocyte apoptosis.
North America western blotting market would grow at 3.80% of CAGR throughout the forecast duration 2019-2027.
Technavio's analysts forecast the global western blotting market to grow at a CAGR of 5.84% during the period 2016-2020.
[alpha]-Tubulin is used as a loading control for Western blotting. Open bars, p75NTR; solid bars, CRABP1.
Nevertheless, the need for facilitated screening of antibody-producing hybridomas in the late 1970s gave rise to Western blotting, again validating the Swiftian dictum of necessity leading to invention.
Western blotting and cross-absorption procedures were performed (15-17).
For Western blotting analysis, the unlavaged lungs were removed, minced into small pieces, snap-frozen in liquid nitrogen, and stored at-80[degrees]C until analyzed for VEGF.
After incubations with various oxygen tensions, cells were immediately processed for western blotting, or immunohistochemistry for the presence of various proinflammatory cytokines.