Western blot

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Related to Western blots: Southern blots, Northern blots
An assay that detects specific proteins within a protein mixture by a multistep process consisting of electrophoresis on a slab gel, transfer of the proteins on the gel to a membrane followed by identification of the specific proteins by antibody staining

Western blot

Immunoblot Molecular diagnostics An assay that IDs antibodies to proteins of specific molecular weights, used to confirm HIV infection–after a positive ELISA screening assay, Lyme disease. See Blot, Electrophoresis, Protein. Cf Northern blot, Southern blot.

West·ern blot a·nal·y·sis

(wes'tĕrn blot ă-nal'i-sis)
A procedure in which proteins separated by electrophoresis in polyacrylamide gels are transferred (blotted) onto nitrocellulose or nylon membranes and identified by specific complexing with antibodies that are either pre- or post-tagged with a labeled secondary protein.
See also: immunoblot
Synonym(s): Western blot, Western blotting.
[coined to distinguish it from eponymic Southern blot a.]

Western blot

A technique developed in 1979 that is used to confirm ELISA results. HIV antigen is purified by electrophoresis and attached by blotting to a nylon or nitrocellulose filter. The patient's serum is reacted against the filter, followed by treatment with developing chemicals that allow HIV antibody to show up as a colored patch or blot. If the patient is HIV-positive, there will be stripes at specific locations for two or more viral proteins. A negative result is blank.
Mentioned in: AIDS Tests
References in periodicals archive ?
When the Bartonella IgXSpot test is combined with Bartonella Western Blots, FISH and PCR testing, one can obtain information on the full spectrum of the patient's immune response to the infection and stage of the disease.
this invitation to tender (itt) has been issued by mdc for the supply, delivery and installation of an imaging solution for western blots. the imaging solution for western blots is to be delivered and installed at the mdc premises at alderley par
After the identification of these peptides with tandem-MS, we can now state with certainty that the bands visible on the Western blots are indeed cTnT-derived products.
Antibodies for Western Blot: anti-Nrf2 antibody (ab137550) and anti-MRP2 antibody (ab172630) were from Abcam (USA).
(a) Representative western blots of phospho- [Ser.sup.1177]eNOS and eNOS.
The article "Effects of Atrazine on Estrogen Receptor a- and G Protein-Coupled Receptor 30-Mediated Signaling and Proliferation in Cancer Cells and Cancer-Associated Fibroblasts" [Environ Health Perspect 123:493-499; doi:10.1289/ ehp.1408586] contains corrected Western blots as well as new data.
of California-Davis and a leading advocate for improving the reliability of western blots, hopes that a decade from now most researchers will be using TPN as their normalization method.
Multiplex capability enables scientists to obtain more information in a single Western blot than chemiluminescence imaging or film, while also providing greater dynamic and linear range.
Gingrich, et al., "Multiplex Detection and Quantitation of Proteins on Western Blots Using Fluorescent Probes," BioTechniques 29, 636-642 (2000).
Para la confirmacion de los sueros positivos se utilizo western blot anti-Borrelia Blot IgG (DRG Diagnostics, Marburg, Germany).
Carestream Health (formerly Kodak) offers the Gel Logic Imaging systems from the entry-level Gel Logic 100 system for colorimetric blots, to the more advanced 2200 imaging system that offers high sensitivity imaging of chemilumensent western blots and low-light fluorescence.
Cells were lysed by 3 freeze/thaw cycles in 10 mmol/L Tris, pH 8.0, and used as a virus lysate for preadsorption in ELISA and Western blots. Where indicated, monkeypox and vaccinia viruses were also purified by ultracentrifugation through 36% sucrose at 40,000 x g for 80 min, followed by band extraction after 25%-40% sucrose gradient sedimentation (33,000 x g for 40 min).