Western blot test

Wes·tern blot test

(wes'tĕrn blot test)
A serum electrophoretic analysis used to identify proteins.

Western blot test

A technique for analyzing protein antigens. Initially, the antigens are separated by electrophoresis on a gel and transferred to a solid membrane by blotting. The membrane is incubated with antibodies, and then the bound antibodies are detected by enzymatic or radioactive methods. This method is used to detect small amounts of antibodies.
References in periodicals archive ?
The antibody labelling services used very effectively in different diagnostic procedures such as western blot test, ELISA, radioimmunoassay, enzyme immunoassay, etc.
Cases in which FIV is suspected based upon ELISA tests can be confirmed by a Western blot test, which demonstrates specific viral proteins in the blood.
To perfect the existing provoked PCR testing procedure, we first evaluated patients with chronic myofascial pain who tested negative or "indeterminate" with the Western blot test. We used manual ART testing with diluted microbial cultures to detect if (and where) in the body we can find resonance or evidence of the presence of specific pathogens.
Testing included screening ELISA, which was positive for IgM antibody, confirmed by IgM positive Western blot test (IgM positive for 39, 41; IgG positive for 23 and 41).
Western blot test results donors with positive ELISA repeatable to disaggregated by sex Positive Unclear P value 0/02 Percent Number Percent Number Man 48 152 23 87 Woman 10 120 1/5 59 Total 58 272 24/5 149 Negative P value 0/02 Percent Number Man 17/5 120 Woman 2/5 58 Total 20 178 Table 2.
McCleary claims the Western blot test is faulty because the CDC is not using two "most Lyme specific'' bands (31 kDa and 34 kDa) in its test.
Eighteen days later, the woman attempted to sell plasma but was refused because she tested positive for HIV by EIA serology screening followed by an HIV-1 Western blot test. On July 5, results of repeated EIA and Western blot tests conducted on the woman at a health clinic were positive for HIV infection.
Due to its small size, the peptide cannot be used in Western blot test, because it diffuses when it is imposed to high voltage.
Group 1 consisted of 344 participants of unknown HIV status; Group 2 consisted of 153 participants previously confirmed with a Western Blot test to be HIV-positive.
"There is a simple test called the Western blot test which is more specific, but it doesn't exist in Ireland.
A follow-up Western blot test six months after initial presentation was positive and included the p31 band.