Western blot test

Wes·tern blot test

(wes'tĕrn blot test)

A serum electrophoretic analysis used to identify proteins.

Medical Dictionary for the Health Professions and Nursing © Farlex 2012

Western blot test

A technique for analyzing protein antigens. Initially, the antigens are separated by electrophoresis on a gel and transferred to a solid membrane by blotting. The membrane is incubated with antibodies, and then the bound antibodies are detected by enzymatic or radioactive methods. This method is used to detect small amounts of antibodies.

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The antibody labelling services used very effectively in different diagnostic procedures such as western blot test, ELISA, radioimmunoassay, enzyme immunoassay, etc.

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Cases in which FIV is suspected based upon ELISA tests can be confirmed by a Western blot test, which demonstrates specific viral proteins in the blood.

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To perfect the existing provoked PCR testing procedure, we first evaluated patients with chronic myofascial pain who tested negative or "indeterminate" with the Western blot test. We used manual ART testing with diluted microbial cultures to detect if (and where) in the body we can find resonance or evidence of the presence of specific pathogens.

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Eighteen days later, the woman attempted to sell plasma but was refused because she tested positive for HIV by EIA serology screening followed by an HIV-1 Western blot test. On July 5, results of repeated EIA and Western blot tests conducted on the woman at a health clinic were positive for HIV infection.

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A follow-up Western blot test six months after initial presentation was positive and included the p31 band.

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