Western blot test

Western blot test

a laboratory blood test to detect the presence of antibodies to specific antigens. It is regarded as more precise than the enzyme-linked immunosorbent assay (ELISA) and is sometimes used to check the validity of ELISA tests.

Wes·tern blot test

(wes'tĕrn blot test)
A serum electrophoretic analysis used to identify proteins.

Western blot test

A technique for analyzing protein antigens. Initially, the antigens are separated by electrophoresis on a gel and transferred to a solid membrane by blotting. The membrane is incubated with antibodies, and then the bound antibodies are detected by enzymatic or radioactive methods. This method is used to detect small amounts of antibodies.
References in periodicals archive ?
Limited Tenders are invited for Procurement Of Western Blot Test (Pvms 270903)
Unfortunately, the "gold standard" western blot test for Lyme disease will miss Lyme 40% to 50% of the time, so it is no better than a coin toss
McCleary claims the Western blot test is faulty because the CDC is not using two "most Lyme specific'' bands (31 kDa and 34 kDa) in its test.
Eighteen days later, the woman attempted to sell plasma but was refused because she tested positive for HIV by EIA serology screening followed by an HIV-1 Western blot test.
By using nanotechnology, gold nanoparticles were used in this research to hold the peptide in the surface of the sheet of Western blot test.
There is a simple test called the Western blot test which is more specific, but it doesn't exist in Ireland.
A follow-up Western blot test six months after initial presentation was positive and included the p31 band.
The confirmatory Western Blot test uses a 1:50 dilution.
The APTIVA assay could be used as a "potential alternative" to the traditional Western blot test that is currently used to confirm HIV-1 infection in a patient with positive HIV-1 antibody screening tests, according to the FDA statement.
The Western blot test, conducted at Weybridge, destroys normal proteins in the brain tissue, leaving only the abnormal prions.
Testing for HIV-2 is not routinely performed due to the low incidence, and only the Western Blot test will find it.
On June 10, USDA announced that three animals that tested negative for BSE last year were retested using the Western Blot test.