Western blot analysis


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Related to Western blot analysis: immunoblot

West·ern blot a·nal·y·sis

a procedure in which proteins separated by electrophoresis in polyacrylamide gels are transferred (blotted) onto nitrocellulose or nylon membranes and identified by specific complexing with antibodies that are either pretagged or posttagged with a labeled secondary protein.
See also: immunoblot.
[coined to distinguish it from eponymic Southern blot a.]

Western blot analysis

n.
A technique for identifying specific antibodies or proteins in which proteins are separated by electrophoresis, transferred to nitrocellulose, and reacted with antibody.

West·ern blot a·nal·y·sis

(wes'tĕrn blot ă-nal'i-sis)
A procedure in which proteins separated by electrophoresis in polyacrylamide gels are transferred (blotted) onto nitrocellulose or nylon membranes and identified by specific complexing with antibodies that are either pre- or post-tagged with a labeled secondary protein.
See also: immunoblot
Synonym(s): Western blot, Western blotting.
[coined to distinguish it from eponymic Southern blot a.]

West·ern blot a·nal·y·sis

(wes'tĕrn blot ă-nal'i-sis)
A procedure in which proteins separated by electrophoresis in polyacrylamide gels are transferred (blotted) onto nitrocellulose or nylon membranes and identified by specific complexing with antibodies that are either pre- or posttagged with a labeled secondary protein.
Synonym(s): Western blot, Western blotting.
References in periodicals archive ?
We performed Western blot analysis by using mAb 3F4 (for detection of human Pr[P.sup.res]) and mAb 6H4 (for detection of CWD Pr[P.sup.res] and human Pr[P.sup.res]).
(b) Western blot analysis shows cyclinD1, p21, and c- Myc protein levels in cancer cells.
Supplemental Figure S1: Western blot analysis of the expression of FGFR2-IIIb and NRP1 in ASCs untreated or treated with KGF for 5;, 15;, 30;, 1 h, 2 h, and 3 h.
We also examined protein expression of IL-1[beta] and MMP-13 under CTS for 24 h using Western blot analysis. As shown in Figure 5, the addition of Sema3A significantly and dose-dependently inhibited expression of IL-1[beta] and MMP-13.
Western Blot Analysis. The cells were treated as mentioned above and lysed in cell lysis buffer (Beyotime Institute of Biotechnology, Shanghai, China) containing 1 [micro]M phenylmethylsulfonyl fluoride, 1.5 [micro]M pepstatin A, and 0.2 [micro]M leupeptin.
The detection of [PrP.sup.Sc] mimics the established procedures used for definitive diagnosis of CJD from brain tissue, whereby an aliquot of the PMCA product after each cycle is digested with proteinase K and subjected to Western blot analysis for detection of PrP.
Western blot analysis revealed that low-dose rapamycin could significantly decrease the expression of senescence markers p16 and p21 in contrast to the ox-LDL group (Figures 5(a) and 5(b)).
Caption: Figure 3: Western blot analysis for caspases and PARP-1 cleavages after curcumin treatments in Hep3B cells.
TLR4 and nuclear NF-[kappa]B p65 expression in H9C2 cells and primary cardiomyocytes of the control and H/R groups were measured by Western blot analysis. As shown in Figures 2(a)-2(d), both TLR4 and nuclear NF-[kappa]B p65 protein levels increased significantly in the H/R group compared to the control group, and this effect was mitigated by DEX pretreatment.
Western blot analysis and immunofluorescence staining for MBP, a marker for mature oligodendrocytes, showed that MBP expression was higher in the NAM treated group than that in the other three groups in the peri-infarct area at 14 d (Figures 2(c), 2(d), and 2(e) and Supplementary Figure 2), further supporting our MRI results.
Western Blot Analysis. Whole-cell extracts were subjected to gel electrophoresis and transferred to a polyvinylidene fluoride membrane.