Western blot

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An assay that detects specific proteins within a protein mixture by a multistep process consisting of electrophoresis on a slab gel, transfer of the proteins on the gel to a membrane followed by identification of the specific proteins by antibody staining
Segen's Medical Dictionary. © 2012 Farlex, Inc. All rights reserved.

Western blot

Immunoblot Molecular diagnostics An assay that IDs antibodies to proteins of specific molecular weights, used to confirm HIV infection–after a positive ELISA screening assay, Lyme disease. See Blot, Electrophoresis, Protein. Cf Northern blot, Southern blot.
McGraw-Hill Concise Dictionary of Modern Medicine. © 2002 by The McGraw-Hill Companies, Inc.

West·ern blot a·nal·y·sis

(wes'tĕrn blot ă-nal'i-sis)
A procedure in which proteins separated by electrophoresis in polyacrylamide gels are transferred (blotted) onto nitrocellulose or nylon membranes and identified by specific complexing with antibodies that are either pre- or post-tagged with a labeled secondary protein.
See also: immunoblot
Synonym(s): Western blot, Western blotting.
[coined to distinguish it from eponymic Southern blot a.]
Medical Dictionary for the Health Professions and Nursing © Farlex 2012

Western blot

A technique developed in 1979 that is used to confirm ELISA results. HIV antigen is purified by electrophoresis and attached by blotting to a nylon or nitrocellulose filter. The patient's serum is reacted against the filter, followed by treatment with developing chemicals that allow HIV antibody to show up as a colored patch or blot. If the patient is HIV-positive, there will be stripes at specific locations for two or more viral proteins. A negative result is blank.
Mentioned in: AIDS Tests
Gale Encyclopedia of Medicine. Copyright 2008 The Gale Group, Inc. All rights reserved.
References in periodicals archive ?
Our western blot analyses showed no change in striatal expression of DDC or VMAT-2, suggesting that dopamine release may not be responsible for the dopaminergic effect of zonisamide.
Consistently, the results of qPCR and Western blot analyses showed that Rab27b expression in small LUAD samples was statistically upregulated than that in noncancerous tissues.
Western blot analyses were performed to detect the effectiveness of FMOD-siRNA on RPE cells.
Results of Western blot analyses were the same as histological analyses (Figure 2(b)).
Western blot analyses of conditioned media provided strong inhibition of OPN release after treatment with XAPC7-TAT and HBX-TAT at 5 [micro]M and 10 [micro]M concentrations, respectively (Figures 6(a) and 6(b)).
Western Blot Analyses. Whole cell protein extracts were prepared from treated cells with radioimmunoprecipitation assay buffer containing 150 mM NaCl, 50 mM Tris, 0.1% sodium dodecyl sulphate, 1% Nonidet P-40, and 0.5% deoxycholate supplemented with protease inhibitor phenyl-methylsulfonyl fluoride.
Western blot analyses were also performed with the C-terminal SAF84 antibody to identify possible additional C-terminal [PrP.sup.res] cleavage products, as described for the CH1641 experimental isolate (17).
Western blot analyses revealed that cadmium markedly increased endogenous bcl-2 protein (to 3-4 times the level in wild-type cells) earlier than metallothionein induction, but that the metal did not enhance the induction of bax, bad, or bcl-xS proteins.
Our study also demonstrates that CD117 is expressed in a subset of endometrial carcinomas using both immunohistochemistry and Western blot analyses. We feel these findings provide a solid foundation for now addressing an important question: Does c-kit play a role in endometrial carcinogenesis?
Western blot analyses of G-proteins in Y-organs and ganglia are in p rogress.
The Western blot analyses and immunohistochemistry provide four lines of evidence that the protein that cross-reacts with the two monoclonal antibodies is homologous to known rhodopsins.