Western blot


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An assay that detects specific proteins within a protein mixture by a multistep process consisting of electrophoresis on a slab gel, transfer of the proteins on the gel to a membrane followed by identification of the specific proteins by antibody staining
Segen's Medical Dictionary. © 2012 Farlex, Inc. All rights reserved.

Western blot

Immunoblot Molecular diagnostics An assay that IDs antibodies to proteins of specific molecular weights, used to confirm HIV infection–after a positive ELISA screening assay, Lyme disease. See Blot, Electrophoresis, Protein. Cf Northern blot, Southern blot.
McGraw-Hill Concise Dictionary of Modern Medicine. © 2002 by The McGraw-Hill Companies, Inc.

West·ern blot a·nal·y·sis

(wes'tĕrn blot ă-nal'i-sis)
A procedure in which proteins separated by electrophoresis in polyacrylamide gels are transferred (blotted) onto nitrocellulose or nylon membranes and identified by specific complexing with antibodies that are either pre- or post-tagged with a labeled secondary protein.
See also: immunoblot
Synonym(s): Western blot, Western blotting.
[coined to distinguish it from eponymic Southern blot a.]
Medical Dictionary for the Health Professions and Nursing © Farlex 2012

Western blot

A technique developed in 1979 that is used to confirm ELISA results. HIV antigen is purified by electrophoresis and attached by blotting to a nylon or nitrocellulose filter. The patient's serum is reacted against the filter, followed by treatment with developing chemicals that allow HIV antibody to show up as a colored patch or blot. If the patient is HIV-positive, there will be stripes at specific locations for two or more viral proteins. A negative result is blank.
Mentioned in: AIDS Tests
Gale Encyclopedia of Medicine. Copyright 2008 The Gale Group, Inc. All rights reserved.
References in periodicals archive ?
It turned out that "every single patient with a" Liaison value of 9 RLUs or higher was confirmed on western blot for Lyme.
Cell lysates were subjected to Western blot. SFigure.4.
Then the LC3, Beclin-1, and p62 expressions in the NP cells were examined and compared by western blot again.
In our study, Western blot assays of non-PD control or PD patient urine samples detected OxiDJ-1, but there was no significant difference in the amount of OxiDJ-1 between PD and non-PD participants (Figures 3(a) and 3(b)).
The 37-kDa protein band was detected as cTnT in 3 of the 12 patients using Western blot, but could only be identified as intact cTnT in 3 samples belonging to patient No.
Moreover, overexpressed HBD HeLa shR cells were unable to induce downregulation of HBD with PHZ treatment, as estimated by Western blot analysis (Figure 7).
Western Blots. Whole cell lysates from treated cells were prepared as previously described.
The bioexpression of PKC[delta] from the cytoplasm to membrane was determined by Western blot analysis.
At the 2010 conference, Bernard Branson, MD, then Associate Director of HIV Laboratory Diagnostics, said that at the time about 20 percent of those tested in the United States never obtained Western blot confirmatory results.
"The CDC needs to recognize the bands for the Western blot that are Lyme disease-related and they're not doing that,'' Mrs.
Levels of amyloid [beta]-peptide (A^) deposits in the hippocampus and cortex were evaluated by immunofluorescence, western blot and enzyme-linked immunosorbent assay.
Key words: Mycoplasma gallisepticum CRD SDS-PAGE Polypeptide and Western blot.