Warburg manometer

Warburg manometer

a RESPIROMETER designed by the German biochemist Otto Warburg, that measures aerobic respiration rates in tissues and small organisms. Carbon dioxide released from respiring material inside a flask is absorbed by potassium hydroxide, the resulting drop in pressure being measured by a manometer. The amount of CO2 produced is equivalent to oxygen uptake by the tissue assuming a RESPIRATORY QUOTIENT of 1.0. The respirometer apparatus is very sensitive to temperature, which is controlled by immersing the flask in a water bath whose temperature is regulated by a very accurate thermostat. Variations in atmospheric pressure do not affect the results since the respirometer is a closed-circuit system.
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