Plotkin and Koprowski would do just that: in 1969, they developed the RA27/3 rubella vaccine, the first of many vaccines using WI-38 cells.
Hayflick's career, however, was tarnished by his involvement in a protracted dispute with the Wistar Institute and the National Institutes of Health over claims to the WI-38 cells. Though Hayflick had been party to agreements stating that he did not have exclusive rights to the WI-38 cells, he insisted that their rightful ownership was in question.
 showed that the cell proportion of the S phase (13.56%) was obviously reduced, whereas the cell proportion of the G1 phase (85.20%) was obviously greater compared with normal control cells with S phase fraction of 21.82% and G1 phase fraction of 61.94% after the WI-38 cells were treated with [H.sub.2][O.sub.2].
Ren, "Guiqi polysaccharide protects the normal human fetal lung fibroblast WI-38 cells from [H.sub.2][O.sub.2]-induced premature senescence," International Journal of Clinical and Experimental Pathology, vol.
MRC-5 and WI-38 cells
were cultured in Minimum Essential Medium Eagle with L-glutamine and sodium bicarbonate (Sigma-Aldrich, St.
were fixed with BD Cytofix/Cytoperm solution for 30 min and then incubated with specific first antibody or isotype control for 30 min at 4[degrees]C in the dark.
We obtained RV16, expanded by infection of WI-38 cells
(ATCC), from David Proud at Johns Hopkins Asthma and Allergy Center.
Using human fibroblast WI-38 cells
and the transformed WI-38RA cells, this study explored apoptosis levels as well as cell viability using Hoechst/propidium iodide staining following treatment with reovirus for 2, 8, 24, 48, and 72 h.
WI-38 cells are considered to be young below 30 population doublings (PDs) and enter senescence at 45 PDs or above.
WI-38 cells submitted to the different treatments were washed with PBS and scrapped on ice in a lysis buffer (10 mM Tris, pH 7.4, 100 mM NaCl, 1mM EDTA, 0.1% Triton X-100) supplemented with protease inhibitors cocktail (Sigma-Aldrich).
In contrast to HL-60 cells, treatment of WI-38 cells
with capillin did not induce DNA fragmentation.
are young, with less than 30 PDs, and enter senescence at 45 PDs or above.