The gut and other internal organs have formed in the visceral cavity, and some larvae possess the remains of a tail, which protrudes as a small stump from the trunk (not shown).
RAI 3 larvae show additional deletions of structures in the forebrain and midbrain-hindbrain border region, severe truncation of the axis, expansion of the visceral cavity, a thin tubular heart, and malformations of the cartilages of the neurocranium and anterior gill arches ([ILLUSTRATION FOR FIGURE 5C OMITTED] and Table III).
RAI 3 Partial deletion of forebrain (FB); broad deletion of MB/HB border region; eyes smaller, closer together, and ventrally and anteriorly rotated; otocysts shifted anterior; axis severely truncated; curled tail; visceral cavity expanded; thin tube heart with contractions but no circulation; blood cells pooled posteriorly; deletions of the quadrate, pterygoid, hyosymplectic, orbital, epiphyseal, and basibranchial cartilage; malformations or deletion of Meckel's cartilage; malformation of the ethmoid, ceratohyals, basihyal, auditory capsule, basibranchials, hypobranchials, and ceratobranchials.
For the study of fecundity, in accordance with Vazzoler (1996), ovaries in stage C were extracted from the visceral cavity and weighed; their membranes were then sectioned longitudinally throughout and immersed in Gilson solution.
In order to test the hypothesis in which repletion and accumulated fat degrees in the visceral cavity depend on the period of the year, a contingency table was made, and the values were tested using the [chi square] test to verify the hypothesis of independence of the two variables categorised (Vieira, 2004), [alpha] = 0.
Variation in repletion degrees and accumulated fat in visceral cavity
The frequencies of different repletion degrees and accumulated fat in the visceral cavity during drought and rainy season times are presented in Figure 4.