Veto Cell

An immune cell which, when recognised by a T cell, induces that T cell’s inactivation. Veto activity occurs in BM, spleen, thymus, foetal liver, and in athymic nude mice, and provides specific suppressor activity, repressing host rejection. It is possible that veto cell suppression mechanisms may be manipulated to prevent allograft rejection
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Amplified fragments of HMPV DNA derived from the Veto cell culture supernatant were subjected to direct sequencing.
Comparison of Veto cell plaque assay, TaqMan reverse transcriptase polymerase chain reaction RNA assay, and VecTest antigen assay for detection of West Nile virus in field-collected mosquitoes.
To assess virus viability in grinding solution, 50 [micro]L of each tested grinding solution and virus dilution was placed into 450 [micro]L of BA-1 diluent, and aliquots of 200 [micro]L of this mixture were added to Veto cells reared on standard nutrient medium (i.
Negative antigen (uninfected Veto cell lysate produced as described above) was placed in every third column of the plate (i.
In the morning, 100 [micro]L of each serum-virus mixture was added onto confluent monolayers of Veto cells and allowed to adsorb at 37[degrees]C for 1 h.
Virus stocks were prepared in Veto cells, and each animal was inoculated with approximately 1,000 PFU.
50]]) prepared by two low-multiplicity passes in Veto cells.
Isolation of SARS-CoV was performed with Veto cells maintained in Dulbecco's modified Eagle medium (D-MEM, BioWhittaker, Verviers, Belgium) and supplemented with 10% fetal calf serum (FCS, HyClone, Perbio Science Erembodegem-Aalst, Belgium), penicillin/streptomycin (BioWhittaker), and 2.
Confluent Veto cells in 6 well plates (Falcon) were incubated in duplicate with 1 mL of PBS containing 100 [micro]L of SARS-CoV HSR1 viral stock in 10-fold serial dilutions from -10e2 (1/100) to -10e8 (1/100,000,000).
If no CPE was observed, aliquots of the first passage were transferred to a second preformed monolayer of Veto cells (FHM, WSS, EPC, and CCO cells in 2001) on day 7.