One day before infection, Vero cells
were seeded onto a 96-well culture plate at a concentration of 2 x 104 cells per well.
Nevertheless, virus isolation from blood-cell fraction was successful in Vero cells
infected for 48 h (isolate RJ-IB1 from the girl and RJ-IB5 from the man).
were grown in the 75 cm2 cell culture flasks (Karrel Flasks, Corning, USA) that contained sterilized DMEM with 10% fetal bovine serum (FBS).
, used to measure the antiviral activity against HSV-1 and bovine herpes virus (BHV), were originally purchased from ATCC.
Cytotoxicity was evaluated by MTT assay in Vero cells
. Cones and leaves ethanol extracts exhibited an interesting activity with [IC.sub.50] of 0.50 and 0.66 mg/ml, respectively, at non-cytotoxic concentration.
are used for the isolation of virus from clinical samples and for virus propagation and titration and virus neutralization studies.
Anti Rabies Vaccine: Inactivated purified rabies vaccine prepared in vero cells
(African green monkey kidney cell line) were obtained from American Type Culture Collections, Rockville, MD.
The filtered samples positive for PEDV variant were inoculated onto Vero cells
. After 3 serial passages, no obvious cytopathic effect in Vero cells
The cytotoxicity of bacterial samples for Vero cells
was determined as previously described (16,17).
A total of 50,000 Vero cells
were grown in 8 central wells of a 24-well cell culture cluster (Corning Incorporated, Corning, NY, USA) with minimal essential medium (MEM; Biowest, Nuaille, France) supplemented with 10% fetal bovine serum (Biowest) and incubated at 37[degrees]C with 5% C[O.sub.2] for 48 hours to obtain 95% confluence.
The clinical samples were inoculated onto Vero cells