sapiens-tc/GIN/2014/Makona-C05 virus (Rocky Mountain Laboratory, National Institutes of Health, Hamilton, MT, USA) was used to generate passage 2 virus stock in
Vero E6 cells (Table 1).
22 pm), diluted serially (10-fold) and titrated in
Vero E6 cells in quadruplicate.
In
Vero E6 cells, which had been inoculated with the specimen, diffuse cytopathic effects typical for DENV-1 developed but at a much later time than for DENV-1-positive plasma specimens from other patients; this finding raised the possibility that DENV-1 had either mutated to reduced replication fitness or that the cells were co-infected with >2 incompatible viruses that were interfering with the replication of each other.
Virus stocks were grown in
Vero E6 cells and titrated by using a 50% tissue culture infectious dose ([TCID.
These 2 viruses were propagated on
Vero E6 cells, titered on
Vero E6 cells, and stored in liquid nitrogen.
No direct antiviral effect for lamivudine was observed at concentrations [less than or equal to] 320 [micro]mol/L in
Vero E6 cells (Table).
To isolate SFTSV, we inoculated subconfluent mono layers of
Vero E6 cells with the RT-PCR-positive semm.
Virus isolation in
Vero E6 cells was attempted from 7 positive nasal swab and fecal specimens that had >106 copies/mL in the original sample in the UpE RT-PCR.
If positive, a second specimen was tested by RT-PCR, inoculated into
Vero E6 cells, and evaluated by IHC.
We observed cytopathic effects in tissues during additional passages in chicken embryo fibroblasts, Muscovy duck embryo fibroblasts, and Vero and
Vero E6 cells.
01 mol/L phosphate-buffered saline; and samples of urine were assayed for arenavirus by cultivation in monolayers of
Vero E6 cells (5).
