sapiens-tc/GIN/2014/Makona-C05 virus (Rocky Mountain Laboratory, National Institutes of Health, Hamilton, MT, USA) was used to generate passage 2 virus stock in Vero E6 cells
22 pm), diluted serially (10-fold) and titrated in Vero E6 cells
In Vero E6 cells
, which had been inoculated with the specimen, diffuse cytopathic effects typical for DENV-1 developed but at a much later time than for DENV-1-positive plasma specimens from other patients; this finding raised the possibility that DENV-1 had either mutated to reduced replication fitness or that the cells were co-infected with >2 incompatible viruses that were interfering with the replication of each other.
Virus stocks were grown in Vero E6 cells
and titrated by using a 50% tissue culture infectious dose ([TCID.
These 2 viruses were propagated on Vero E6 cells
, titered on Vero E6 cells
, and stored in liquid nitrogen.
No direct antiviral effect for lamivudine was observed at concentrations [less than or equal to] 320 [micro]mol/L in Vero E6 cells
To isolate SFTSV, we inoculated subconfluent mono layers of Vero E6 cells
with the RT-PCR-positive semm.
Virus isolation in Vero E6 cells
was attempted from 7 positive nasal swab and fecal specimens that had >106 copies/mL in the original sample in the UpE RT-PCR.
If positive, a second specimen was tested by RT-PCR, inoculated into Vero E6 cells
, and evaluated by IHC.
We observed cytopathic effects in tissues during additional passages in chicken embryo fibroblasts, Muscovy duck embryo fibroblasts, and Vero and Vero E6 cells
01 mol/L phosphate-buffered saline; and samples of urine were assayed for arenavirus by cultivation in monolayers of Vero E6 cells