VYS cells from the diabetic group showed a similar number of viable cells with active mitochondria (88.14 [+ or -] 1.6) compared with the control group (94.1 [+ or -] 1.3).
The number of diploid VYS cells was not different at gd 15 in the diabetic group (51.3 [+ or -] 6.3) compared with the controls (62.9 [+ or -] 5.9).
The expression of cell markers CD90, STRO-1 and CD44 on VYS cells in both the control and diabetic groups confirmed the presence of MSCs in the rat VYS.
Reduced OCT3/4 and CD34 expression was detected on VYS cells in the diabetic group while Nanog expression was not different between groups.
The immunopositivity for CD117, a marker for hematopoietic cell precursors, suggests that VYS cells at gd 15 retain the potential to generate hematopoietic lineages.
An increased number of VYS cells with inactive mitochondria (unviable cells) was present in the diabetic group.