Therefore, the proposed assay can quantify the VLDLR and IDL fractions, which reflect CAD progression, as reported in the Montreal Heart Study (6).
9] Nonstandard abbreviations: TRL, triglyceride-rich lipoprotein; CAD, coronary artery disease; VLDLR, VLDL remnant; IDL, intermediate-density hpoprotein; RLP-C, remnant-like particle cholesterol; apo, apolipoprotein; CM, chylomicron; LPL, lipoprotein lipase; CMR, CM remnant; TG, triglyceride; PL-D, phospholipase D; CHER, cholesterol esterase; CHOD, cholesterol oxidase; POE-POB, polyoxyethylene-polyoxybutylene block copolymer.
We examined 180 surfactants with diverse structures, with which selectivity toward cholesterol in VLDLRs was investigated.
Using gel filtration and size distribution studies, we gained insight into the mechanism by which POE-POB exhibits its selectivity toward VLDLRs.
The proposed assay measures cholesterol in CMRs, VLDLRs, and IDLs by selectively modifying these 3 remnant lipoproteins with POE-POB and PL-D in the presence of CHER and CHOD.
Because there are no fundamental differences in the overall structure of CMs, VLDLs, CMRs, and VLDLRs, however, it has been difficult to differentiate these lipoproteins.