HMGB1, TLR4, phospho-NF-[kappa]B (p-NF-[kappa]B), and VE-cadherin
expression in LPS-stimulated HPMEC cells
Delayed vasculogenesis and impaired angiogenesis due to altered Ang-2 and VE-cadherin
levels in the chick embryo model following exposure to cadmium.
, which is only expressed in the epithelial cell, and JAM-1 are key factors in vascular hemostasis and angiogenesis [5, 6].
PB-MNC, stimulated or not with ephrin-B2, did not modify Evans blue extravasation (N = 6 - 7) or occludin, claudin-5, ZO-1, or VE-cadherin
mRNA expression compared to PBS (N =5 - 7) (Figure 5).
Adherens junctions are a major part of the complex, in which VE-cadherin
, an endothelium-specific component of adhesion proteins, controls both adherens junctions and endothelial barrier integrity [20, 21].
The protective role of Sirt1 in LPS-induced EC hyperpermeability was then verified by monitoring the monolayer barrier and detecting the morphological alterations of F-actin and VE-cadherin
. It was revealed that the decrease in TER level induced by LPS was remarkably reversed by both pretreatment and simultaneous treatment with SRT1720, consistent with the reversed decrease in flux of dextran (Figure 3(a)).
Further activation of Src was mediated by tumor cell-derived IL8 and VLA-4/VCAM-1 interactions leading to phosphorylation of VE-cadherin
, a major component of endothelial junctions and finally to disruption of the endothelial barrier .
We measured the HUVEC permeability, proliferation, and the secretion of VE-cadherin
into culture medium using fluorescein isothiocyanate-dextran assay, 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide assay, and enzyme-linked immunosorbent assay, respectively, in response to treatment with MMP9 and/or rhubarb monomers.
In TGF-[beta]1 stimulated HPMECs, treatment with GA resulted in a reduction of EndoMT with a decrease in vimentin, and an increase in VE-cadherin
In addition, [beta]-cadherin is found in the placenta (36), VE-cadherin
is found in the vascular endothelium, and R-and K-cadherin are found in the retina and kidney, respectively (38).