An upregulation of VCAN was also observed after 7, 14, 21, and 28 days of chondrogenic stimulation (Figure 3(a)), while no amplification was detected for mRNA corresponding to COL2A1 and ACAN (Figure 3(a)).
VCAN mRNA showed a light time-dependent upregulation, while the expression of ACAN mRNA was significantly increased up to 21 and 28 days of chondrogenic differentiation (Figure 3(b)).
In the DPMSCs, while the transcription factor SOX9 and VCAN had an increase of expression, there was no expression of COL2A1 and ACAN.
Another important system for the interior of Northeast Brazil is VCANs which form over the South Atlantic Ocean and have an impact near the coast of Northeast Brazil.
In the A3 area, corresponding to eastern Northeast Brazil, the presence of VCANs is also observed; however, their influence depends largely on the location of the center of the vortex.
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Developmental genes such as Bcan, Fbln2, Itih3, Ncam1, Tnr, and Vcan
modulate NSC differentiation via Wnt/[beta]-catenin pathway at early stage of differentiation and TGF-[beta] signaling pathway at later (7 day) stage.