The 3' UTR
miRNA-related SNPs may be within or at the vicinity of the miRNA binding site, which could impair regulatory functions of the associated miRNA [18,19].
The 5' untranslated region (5' UTR
) of messenger RNA has been shown to perform an important regulatory function in post-transcriptional processes (19).
In brief, we purified HPgV 5' UTR
and HPgV-E2 PCR products by using the Wizard PCR Preps DNA Purification System (Promega, Madison, WI, USA).
Mock miRNAgene pairs are made for every actual miRNA-3' UTR
of the positive dataset.
A, Sequence alignment of miR-1229-5p and its target sites in 3' UTR
segments of human MAPK1 gene.
In conclusion, miR-125a targeted TRXR1 3'UTR
and resulted in downregulation of endogenous TRXR1 expression in HUVECs.
5' -CCCTGCTTTAATGTATCCTT- 3' (reverse) was designed to amplify the 3'UTR
of SPP1 gene in riverine buffalo.
For example, an SNP is located 14 bp downstream of the miR-24 target site in DHFR 3 UTR
, does not directly fall within the miRNA target set, and resulted in DHFR overexpression and MTX resistance .
Among 51,090 identified poly (A) sites, 54.9% of PAS were mapped to the known UCSC TTS sites, 18.34% mapped to poly (A) database, and 15.4% mapped to the known 3' UTR
region which may be the novel PAS, as shown in Figure 3(b).
From the NCBI (rs645641082) database, the 16-bp indel was predicted to be in the 5' UTR
of the ZNF132 gene in the Yunnan black goat.