UGP gene expression and UDP-glucose
pyrophosphorylase enzymatic activity in grafting annonaceous plants.
pyrophosphorylase (UGP2, gi|183397343) catalyzes the reversible production of UDP-glucose
and pyrophosphate from glucose-1-phosphate and UTP and is a key enzyme for carbohydrate metabolism.
Galactose is the hydrolysate of agar/agarose and is utilized by strain PR1 via the enzyme catalytic activities of galactokinase (GalK), UDP-glucose
4-epimerase (GalE), UDP-glucosehexose-1-phosphate uridylyltransferase (GalT), UTP-glucose-1-phosphate uridylyltransferase (GalU), and phosphoglucomutase (PGM).
The following chemicals and reagents were used: Ampicillin (FLUKA), Isopropyl AY-D-1 thiogalactopyranoside (IPTG; SIGMA), LB broth (SIGMA), XL-1 Blue competent cells (AGILENT TECHNOLOGIES UK LTD), Agar (FLUKA), 1Kb DNA ladder (NEW ENGLAND BIOLABS), PCR nucleotide mix, Taq buffer and Taq polymerase X (PROMEGA), Primers (EUROFINS MWG OPERON), GStrap FF (GE HEALTHCARE), Phenol blue (SIGMA-ALDRICH), 10X Tank buffer (BIO-RAD), N-N-N-N- tetramethylethylenediamine (TEMED) (SIGMA), Ammonium per sulphate (APS) (SIGMA), Sugar donor UDP-Glucose
(Sigma X), novobiocin, kaempferol and quercetin (SIGMA), UDP Rhamnose (a kind gift from Prof.
[25.] Walsh EC, Stainier DYR (2001) UDP-Glucose
dehydrogenase required for cardiac valve formation in zebrafish.
The availability of precursors is also important for controlling HA synthesis since UDP-glucose
pyrophosphorylase and dehydrogenase are known to be necessary for sustaining HA production .
Additionally, Method I has a glycosylation step in which the DNA is previously treated with T4 [beta]-glucosyltransferase and UDP-glucose
. In both methods, the unmodified DNA (C) is digested by HpaII and MspI and the methylated DNA (5-mC) is digested only by MspI.
(2003) Glycopeptide specificity of the secretory protein folding sensor UDP-glucose
Estimation of the enzyme--The estimation of enzyme was performed by taking 0.2 ml of above supernatant to which 0.2 ml HEPES- KOH buffer 0.1 M (pH of 7.5), 0.2 ml sucrose 0.5 M, 0.2 ml UDP 0.01 M, and 0.1 ml UDP-glucose
dehydrogenase were added.
Mock infected Microarray GC LC Oryzasativaisocitrate lyase (ICL1) 1 Citric acid Succinate Glyoxylate Glucose Pyruvate - [beta]-1, 3 glucanase Chitinase - N-Acetyl-D-glucosamine Glutamate decarboxylase (GAD1) + GABA (4-amino butyric acid) Glutamic acid Phenylalanine ammonia-lyase (PAL) Phenylalanine Glutathione S-transferases - Oxidized glutathione - Lipid transfer protein b1 + UDP-glucose
: salicylic acid - glucosyltransferase (SA-GTase) Uridine 5' diphosphate UDP-glucose
Salicylate Gene name & metabolities Transgenic (resistant strain) vs.
In particular, the invention relates to the inhibition of the enzymatic activity of a UDP-glucose
: sinapate glucosyltransferase (SGT) in transgenic plant cells.
It is well known that sucrose hydrolyzes into fructose and glucose by the catalytic action of invertase isoenzymes or converts into uridine diphosphate-glucose (UDP-glucose
) and fructose by sucrose synthase (Avigad and Dey, 1997).