The following chemicals and reagents were used: Ampicillin (FLUKA), Isopropyl AY-D-1 thiogalactopyranoside (IPTG; SIGMA), LB broth (SIGMA), XL-1 Blue competent cells (AGILENT TECHNOLOGIES UK LTD), Agar (FLUKA), 1Kb DNA ladder (NEW ENGLAND BIOLABS), PCR nucleotide mix, Taq buffer and Taq polymerase X (PROMEGA), Primers (EUROFINS MWG OPERON), GStrap FF (GE HEALTHCARE), Phenol blue (SIGMA-ALDRICH), 10X Tank buffer (BIO-RAD), N-N-N-N- tetramethylethylenediamine (TEMED) (SIGMA), Ammonium per sulphate (APS) (SIGMA), Sugar donor UDP-Glucose (Sigma X), novobiocin, kaempferol and quercetin (SIGMA), UDP Rhamnose (a kind gift from Prof.
Ten L 1 mM UDP-glucose, 10 L 1mM novobiocin and 10 L enzyme were added into a sterile eppendorf tube and incubated over night at 37C.
Walsh EC, Stainier DYR (2001) UDP-Glucose
dehydrogenase required for cardiac valve formation in zebrafish.
Additionally, Method I has a glycosylation step in which the DNA is previously treated with T4 [beta]-glucosyltransferase and UDP-glucose
2003) Glycopeptide specificity of the secretory protein folding sensor UDP-glucose glycoprotein:glucosyltransferase.
2003) The non catalytic portion of human UDP-glucose: glycoprotein glucosyltransferase I confers UDP-glucose binding and transferase function to the catalytic domain.
The cellular concentration of UDP-glucuronide is determined by the activity of UDP-glucose
dehydrogenase that catalyzes the conversion of UDP-glucose
08, 797,226, "DNA Sequences Encoding Solanidine UDP-Glucose
Glucosyltransferase and Use to Reduce Glycoalkaloids in Solanaceous Plants," contact William R.
was considered to be channeled to cellulose synthase in the plasma membrane, implying that sucrose availability would affect the rate of the cellulose synthesis.
SPS activity was assayed by quantitation of the fructose6-P dependent formation of sucrose-6-P from UDP-glucose
by a procedure adapted from Huber and Israel (1982).
In the GALE assay, enzymatic conversion from UDP-galactose to UDP-glucose
is detected either by radioactive (9) or nonradioactive assay, with substrates and products separated and quantified by HPLC (11).
For our studies into the role of the hexosamine pathway in type 2 diabetes mellitus, we were interested in a method to assess UDP-galactose (UDP-Gal), UDP-glucose
(UDP-Glc), UDP-GalNAc, and UDP-G1cNAc in small samples of human muscle tissue, e.