Feve, "Current status of tyrosine hydroxylase
in management of Parkinson's disease," CNS and Neurological Disorders--Drug Targets, vol.
Wang et al., "Coexpression of tyrosine hydroxylase
, GTP cyclohydrolase I, aromatic amino acid decarboxylase, and vesicular monoamine transporter 2 from a helper virus-free herpes simplex virus type 1 vector supports high-level, long-term biochemical and behavioral correction of a rat model of Parkinson's disease," Human Gene Therapy, vol.
Isolation and characterization of the gene for drosophila tyrosine hydroxylase
After centrifugation at about 400-600 X g for 5-10 s using a standard benchtop centrifuge, the supernatant was removed, and the tissues were fixed for 4-12 h, either in 4% paraformaldehyde in 0.1 mol [1.sup.-1] phosphate buffer (pH 7.4) at 4[degrees] C for eventual detection of 5-HT or FMRFamide-related peptides, or in methanol at -18[degrees] C for the detection of tyrosine hydroxylase
Brain tissue samples were collected and stained with Tyrosine hydroxylase
. Cell counts and fiber grades were performed on each tissue sample.
Tyler's spinal tap was immediately sent to Keith Hyland's laboratory and a diagnosis of tyrosine hydroxylase
deficiency was made.
The animals were terminated at 16-18 weeks and kidneys and adrenals removed for the estimation of tyrosine hydroxylase
activity by HPLC.
One pterin, tetrahydrobiopterin (BH4), is a necessary cofactor for tyrosine hydroxylase
(TH) which is involved in neurotransmitter biosynthesis.
Pathologic studies on the brains of 2 patients who died from causes unrelated to their transplants indicated that both had "robust survival of tyrosine hydroxylase
immunuoreactive cells and abundant reinnervation of the postcommissural putamen," Hauser and colleagues wrote.
In comparing the NGF-mediated induction of tyrosine hydroxylase
(TH) activity and of neurite outgrowth in cultures of adrenal chromaffin cells from fetal, neonatal, and adult bovine animals, Naujoks et al.
In test-tube studies, the scientists found that the phthalide compound blocks the action of an enzyme called tyrosine hydroxylase
, which the body used to produce catecholamines.
DA is synthesized from the amino acid tyrosine that crosses the blood-brain barrier, is transformed into L-3,4- dihydroxyphenylalanine (L-DOPA) by tyrosine hydroxylase
and then, to DA by dopadecarboxylase.