Las soluciones experimentales fueron utilizadas en las siguientes concentraciones finales: bloqueador de canal lento de [Na.sup.+], amiloride (Sigma), 0.2, 0.02 y 0.002 [micron]M; bloqueador de canal tipo L de [Ca.sup.2+], nifedipina (Sigma), 0.5 [micron]M; solucion activadora del intercambiador [Na.sup.+]/[Ca.sup.2+], Tyrode
conteniendo [Na.sup.+] 80 mM.
The pellets were resuspended into Tyrode
HEPES buffer, pH 7.35, and platelets were counted with a thrombocoulter counter.
After overnight fasting (10-12 h), the rats were anesthetized with diethyl ether and the small intestine at the ileocecal junction was isolated and rinsed with Tyrode
buffer solution pH 7.4 (containing in mM: 15 glucose, 11.90 NaHC[O.sub.3], 136.9 NaCl, 4.2 Na[H.sub.2]P[O.sub.4], 2.7 KCl, 1.2 Ca[Cl.sub.2], and 0.5 Mg[Cl.sub.2]) at room temperature.
Then, hearts were rapidly removed, mounted on a Langendorff perfusion apparatus, and retrogradely perfused with [Ca.sup.2+]-free tyrode
solution composed of (in mM) NaCl 120, KCl 5.4, Mg[Cl.sub.2] 0.5, HEPES 25, and glucose 10 (pH 7.4 with NaOH) at 37[degrees] C.
The intestine was immediately placed in 37 C and oxygenated ([O.sub.2]/[CO.sub.2], 95%:5%) in Tyrode
y a la auxiliar de Secretaria de Redaccion, Estructuracion y Canje, Br.
The rats and guinea pig were killed by a blow on the head dissected for the parts were placed in dishes containing Tyrode
Briefly, PRP containing 5 mM EDTA, 2U/mL apyrase, and 0.1 [micro]g/mL PGE1 was centrifuged at 1100 xg for 10 min, and then pellets were resuspended in Tyrode
buffer and the concentration of platelets was adjusted to approximately 3 x [10.sup.7] platelets/mL.
Acetylcholine, atropine, modified Tyrode
's Salt Solution, and N[omega]-nitro-L-arginine methyl ester hydrochloride were acquired from Sigma-Aldrich Chemical Co.
The hearts were quickly removed and washed with cool and oxygenated Tyrode
Siempre bajo el liderazgo de los Profesores Jose Villalobos e Ingrid Goilo de Tyrode
y otros colegas que no he querido mencionar por temor a dejar alguno de ellos por fuera, pero que he de reconocerlos ante los lectores de la Revista Entre Lenguas por su gran aporte para hacer posible, a lo largo de estos anos, el exitoso proyecto editorial del Centro de Investigaciones de Lenguas Extranjeras de la Facultad de Humanidades y Educacion de la Universidad de Los Andes en Merida, Venezuela.
Each group was divided into two groups for the isolated heart perfusion, according to the perfusion solution utilized (Tyrode