Frozen-thawed spermatozoa washed twice with tyrode
albumin lactate pyruvate medium (TALP, 92.
solution composed of (in mM) NaCl 120, KCl 5.
sup], Under physiological-like condition, that is, in CO [sub]2 /HCO [sub]3[sup]− buffered Tyrode
solution, Na [sup]+ -HCO [sub]3[sup]− -dependent transporter, that is, NBC, has been found to be another acid extruder to accompany NHE to achieve acid equivalent extrusion following intracellular acidosis in many different cell types.
The muscle was then excised and fixed between two platinum plate electrodes in 50 ml Radnotti tissue bath filled with Tyrode
solution (121 mM NaCl, 5 mM KCl, 0.
currents, cardiomyocytes were maintained in a Tyrode
solution, containing (in mM) 140 NaCl, 5.
Los cardiomiocitos aislados se almacenaron en una solucion de Tyrode
(en mM) Glucosa 5,5, NaCl 136,5, KC1 5,4, Mg[Cl.
Solo el grupo control estuvo expuesto una mitad a una cepa de Lactobacillus acidophilus y la otra en solucion tyrode
The muscles were then thoroughly washed in Tyrode
solution (and occasionally maintained in culture medium) and dissociated with fire-polished glass Pasteur pipettes.
Los corazones se extrajeron rapidamente y se sumergieron en solucion Tyrode
fria (10[grados] C) libre de [Ca.
Each semen sample was divided into nine aliquots of 200 ul each, three replicates for each of the two experimental and control group, one of the experimental groups was supplemented with 50ul vitamin-E (a-tocopherol) solution (1mM) in Ringer- tyrode
as described by Verma and Kanwar (1999).
Las plaquetas de una parte del PRP fueron lavadas tres oportunidades con tampon Tyrode
modificado (NaCl 137 mM, KCl 2,8 mM, Mg[Cl.
O pellet foi ressuspendido com 0,5mL do meio Tyrode
S albumin lactate pyruvate (TALP) (PARRISH et al.